Neuberger M S, Calabi F
Nature. 1983;305(5931):240-3. doi: 10.1038/305240a0.
Specific chromosome translocations have been observed in transformed cell lines of both man and mouse and may be implicated in the origin or maintenance of malignancy. In mouse plasmacytomas, translocations have been identified that bring the immunoglobulin alpha heavy-chain gene (C alpha, normally located on chromosome 12) into proximity with c-myc (normally located on chromosome 15), c-myc being the mouse cellular homologue of the avian myelocytomatosis virus transforming gene (v-myc). Here we identify a DNA rearrangement in a mouse hybridoma that has brought c-myc close to C gamma 2b and show that this rearrangement occurred by reciprocal chromosome translocation, as recombinant clones were isolated from the same cell line in which a rearranged variable-region (VH) gene has been brought close to 5' c-myc sequences. The translocation has resulted in the net loss of 7 base pairs (bp) of chromosome 15 sequence as well as in the presence of an additional base of unknown provenance. This reciprocal translocation was analysed in DNA from a mouse hybridoma cell line but is shown to be characteristic of the X63Ag8 myeloma parent.
在人和小鼠的转化细胞系中均观察到了特定的染色体易位，这些易位可能与恶性肿瘤的起源或维持有关。在小鼠浆细胞瘤中，已鉴定出一些易位，这些易位使免疫球蛋白α重链基因（Cα，通常位于12号染色体上）与c-myc（通常位于15号染色体上）靠近，c-myc是禽成髓细胞瘤病毒转化基因（v-myc）的小鼠细胞同源物。在这里，我们在一个小鼠杂交瘤中鉴定出一种DNA重排，该重排使c-myc靠近Cγ2b，并表明这种重排是通过相互染色体易位发生的，因为从与一个重排的可变区（VH）基因靠近5' c-myc序列的同一细胞系中分离出了重组克隆。该易位导致15号染色体序列净丢失7个碱基对（bp），并且还存在一个来源不明的额外碱基。这种相互易位在一个小鼠杂交瘤细胞系的DNA中进行了分析，但显示出是X63Ag8骨髓瘤亲本的特征。
