Eigen M, Lindemann B, Winkler-Oswatitsch R, Clarke C H
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2437-41. doi: 10.1073/pnas.82.8.2437.
Some 200 different 5S rRNA sequences from eubacteria, chloroplasts, mitochondria, archaebacteria, and eukaryotes were analyzed for evolutionary kinship relationships and associated sequential features. Group-specific occupation schemes for the 149 positions of an overall alignment were established. Eubacterial, archaebacterial, and intermediate occupation schemes all yield a strongly biased base triplet pattern in one of the three possible reading frames strongest for eubacterial, chloroplastic, and archaebacterial, but still detectable for mitochondrial and eukaryotic cytoplasmic sequences. The frequency of triplets decays in the order RNY greater than RNR greater than YNY greater than YNR; R being a purine (guanine or adenine), Y is a pyrimidine (cytosine or uracil), and N is any base. A strong preference for guanine or cytosine was found in all triplet positions. The effects show no exceptions and are clearly above the level of statistical fluctuations.
对来自真细菌、叶绿体、线粒体、古细菌和真核生物的约200种不同的5S rRNA序列进行了进化亲缘关系及相关序列特征分析。建立了总体比对中149个位置的组特异性占据模式。真细菌、古细菌和中间型占据模式在三个可能的阅读框之一中均产生强烈偏向的碱基三联体模式，对真细菌、叶绿体和古细菌而言这种模式最强，但对于线粒体和真核细胞质序列仍可检测到。三联体的频率按RNY＞RNR＞YNY＞YNR的顺序衰减；R为嘌呤（鸟嘌呤或腺嘌呤），Y为嘧啶（胞嘧啶或尿嘧啶），N为任意碱基。在所有三联体位置均发现对鸟嘌呤或胞嘧啶有强烈偏好。这些效应无一例外，且明显高于统计波动水平。
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