Küntzel H, Heidrich M, Piechulla B
Nucleic Acids Res. 1981 Mar 25;9(6):1451-61. doi: 10.1093/nar/9.6.1451.
A phylogenetic tree was constructed by computer analysis of 47 completely determined 5S rRNA sequences. The wheat mitochondrial sequence is significantly more related to prokaryotic than to eukaryotic sequences, and its affinity to that of the thermophilic Gram-negative bacterium Thermus aquaticus is comparable to the affinity between Anacystis nidulans and chloroplastic sequences. This strongly supports the idea of an endosymbiotic origin of plant mitochondria. A comparison of the plant cytosol and chloroplast sub-trees suggests a similar rate of nucleotide substitution in nuclear genes and chloroplastic genes. Other features of the tree are a common precursor of protozoa and metazoa, which appears to be more related to the fungal than to the plant protosequence, and an early divergence of the archebacterial sequence (Halobacterium cutirubrum) from the prokaryotic branch.
通过对47个完全确定的5S rRNA序列进行计算机分析构建了系统发育树。小麦线粒体序列与原核生物序列的相关性明显高于与真核生物序列的相关性,并且它与嗜热革兰氏阴性细菌嗜热水生栖热菌的序列亲和力与集胞藻和叶绿体序列之间的亲和力相当。这有力地支持了植物线粒体共生起源的观点。对植物细胞质和叶绿体子树的比较表明,核基因和叶绿体基因中的核苷酸替代率相似。该树的其他特征包括原生动物和后生动物的共同祖先,它似乎与真菌的原序列比与植物的原序列更相关,以及古细菌序列(红嗜盐菌)从原核生物分支的早期分化。