Rylatt D B, Wyatt D M, Bundesen P G
Vet Immunol Immunopathol. 1985 Feb;8(3):261-71. doi: 10.1016/0165-2427(85)90085-6.
A competitive enzyme immunoassay (EIA) for the detection of circulating bovine antibodies to Brucella abortus has been developed using horseradish peroxidase conjugated monoclonal antibodies (MAb) raised against B. abortus cell surface antigens. Antibodies present in the serum of either vaccinated or infected cattle can apparently displace the conjugated MAb from the lipopolysaccharide antigen (LPS) in a quantitatively different manner allowing an assessment of immune status of the animal. The results from a panel of sera from animals with a known status of vaccination or infection indicated that the test was more selective in the detection and discrimination of infected from uninfected or immunized animals, than conventional complement fixation, agglutination or indirect enzyme immunoassay procedures.
一种用于检测牛流产布鲁氏菌循环抗体的竞争性酶免疫测定（EIA）已被开发出来，该方法使用了针对牛流产布鲁氏菌细胞表面抗原产生的辣根过氧化物酶偶联单克隆抗体（MAb）。接种疫苗或感染的牛血清中的抗体显然可以以定量不同的方式从脂多糖抗原（LPS）上取代偶联的单克隆抗体，从而评估动物的免疫状态。一组已知接种或感染状态动物的血清检测结果表明，与传统的补体结合、凝集或间接酶免疫测定方法相比，该检测方法在检测和区分感染动物与未感染或免疫动物方面更具选择性。
