Zhejiang Institute of Landscape Plants and Flowers, Hangzhou, Zhejiang, China.
PeerJ. 2024 Aug 29;12:e17999. doi: 10.7717/peerj.17999. eCollection 2024.
Sucrose invertase is an important catalytic enzyme that is widely distributed in plants and can irreversibly hydrolyze sucrose into fructose and glucose. Daylily is an important perennial flower worldwide and a traditional vegetable in East Asia. Previous studies have suggested that sucrose invertase is involved in the aging of daylily flowers. However, knowledge about the number, physicochemical properties, and expression patterns of daylily sucrose invertases is still lacking. Identifying the daylily sucrose invertase family genes in the genome is highly important for understanding phylogenetic evolution and determining the genetic function of sucrose invertase.
To obtain basic knowledge about the number, classification, sequence composition, and physicochemical properties of sucrose invertases in daylily, bioinformatics software was used to analyze the genome of (), and the basic properties of sucrose invertase genes and proteins were obtained. Then, combined with transcriptome data from flower organs at different developmental stages, the expression patterns of each gene were clarified. Finally, the reliability of the transcriptome data was verified by quantitative real-time polymerase chain reaction (PCR).
Through software analysis, 35 sucrose invertases were identified from the genome and named HcINV1-HcINV35; these enzymes belong to three subfamilies: cell wall invertases, vacuolar invertases, and chloroplast invertases. The amino acid composition, motif types, promoter composition, gene structure, protein physicochemical properties, gene chromosomal localization, and evolutionary adaptability of daylily invertases were determined; these results provided a comprehensive understanding of daylily invertases. The transcriptome expression profile combined with fluorescence quantitative reverse transcription-polymerase chain reaction (RT‒PCR) analysis suggested that almost all daylily invertase genes were expressed in flower organs, but even genes belonging to the same subfamily did not exhibit the same expression pattern at different developmental stages, suggesting that there may be redundancy or dissimilation in the function of daylily sucrose invertases.
蔗糖转化酶是一种广泛分布于植物中的重要催化酶，可将蔗糖不可逆地水解为果糖和葡萄糖。萱草是全球重要的多年生花卉，也是东亚地区的传统蔬菜。先前的研究表明，蔗糖转化酶参与萱草花朵的衰老。然而，有关萱草蔗糖转化酶的数量、理化性质和表达模式的知识仍然缺乏。鉴定萱草蔗糖转化酶家族基因在基因组中对于理解系统发育进化和确定蔗糖转化酶的遗传功能非常重要。
为了获得萱草蔗糖转化酶数量、分类、序列组成和理化性质的基本知识，使用生物信息学软件分析了 基因组，并获得了蔗糖转化酶基因和蛋白质的基本性质。然后，结合不同发育阶段花器官的转录组数据，阐明了每个基因的表达模式。最后，通过定量实时聚合酶链反应（PCR）验证转录组数据的可靠性。
通过软件分析，从 基因组中鉴定出 35 种蔗糖转化酶，并将其命名为 HcINV1-HcINV35；这些酶属于三个亚家族：细胞壁转化酶、液泡转化酶和叶绿体转化酶。确定了萱草转化酶的氨基酸组成、基序类型、启动子组成、基因结构、蛋白质理化性质、基因染色体定位和进化适应性；这些结果提供了对萱草转化酶的全面了解。结合荧光定量逆转录聚合酶链反应（RT-PCR）分析的转录组表达谱表明，几乎所有萱草转化酶基因都在花器官中表达，但即使属于同一亚家族的基因在不同发育阶段也没有表现出相同的表达模式，这表明萱草蔗糖转化酶的功能可能存在冗余或异化。
