绵羊和山羊是多黏菌素耐药大肠杆菌的储主：首次在北非检测到携带 mcr-1 的肠产毒性大肠杆菌 ST10 和大肠杆菌 ST6396 菌株。

Sheep and goats as reservoirs of colistin-resistant E. coli: first detection of ETEC ST10 and E. coli ST6396 mcr-1 positive strains in North Africa.

机构信息

Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Science and Technology - Houari Boumediene, Bab Ezzouar, Algiers 16111, Algeria.

Laboratory ward, National Bone Marrow Transplant Center, 1006 Tunis, Tunisia.

出版信息

J Appl Microbiol. 2024 Sep 2;135(9). doi: 10.1093/jambio/lxae227.

DOI:10.1093/jambio/lxae227

PMID:39223099

Abstract

AIM

This study aimed to screen and characterize colistin-resistant strains isolated from different livestock species in Algeria, including sheep, goats, and dromedaries.

METHODS AND RESULTS

A total of 197 rectal and nasal swabs were screened for colistin-resistant Gram-negative bacilli. Twenty one isolates were selected, identified, and their antibiotic resistance was phenotypically and genotypically characterized. The majority (15/21) were affiliated to Escherichia coli, from which 4 strains isolated from sheep (n = 2) and goats (n = 2) and belonging to phylogroup A and ST10 and ST6396 lineages, respectively, carried the mcr-1 gene. The remaining isolates were identified as belonging to the following genera: Raoultella, Enterobacter, Klebsiella, and Pseudomonas.

CONCLUSION

This study highlights the presence of virulent and multiresistant Gram-negative bacilli in farm animals, increasing the risk of transmitting potentially fatal infections to humans.

摘要

目的

本研究旨在筛选和鉴定来自阿尔及利亚不同家畜（包括绵羊、山羊和单峰驼）的粘菌素耐药菌株，并对其进行特征分析。

方法与结果

共筛选了 197 份直肠和鼻腔拭子，以检测对粘菌素耐药的革兰氏阴性杆菌。选择了 21 株分离株进行鉴定，并对其表型和基因型的抗生素耐药性进行了特征分析。其中大多数（15/21）为大肠杆菌，其中 4 株分离自绵羊（n=2）和山羊（n=2），分别属于 A 群和 ST10 和 ST6396 谱系，携带 mcr-1 基因。其余分离株鉴定为属于以下属：拉乌尔菌属、肠杆菌属、克雷伯菌属和假单胞菌属。