Goswami S K, Gould R M
Biochim Biophys Acta. 1985 Apr 25;834(2):263-6. doi: 10.1016/0005-2760(85)90164-x.
The transformation of [1-14C]arachidonic acid into radiolabeled prostanoids was studied with homogenates and desheathed sciatic nerves of rats and frogs. All of the preparations studied were shown to synthesize prostaglandins; the specific prostanoids made were characterized by their migration on thin-layer chromatograms in three separate solvent systems. Both desheathed rat nerve and homogenates synthesize prostaglandin E2, prostaglandin F2 alpha, prostaglandin D2, 6-ketoprostaglandin F1 alpha and thromboxane B2. With preparations from frog nerve, prostaglandin E2 was the major prostanoid product formed. Several conditions were able to modulate the production of prostaglandin E2 with desheathed frog nerve. Electrical stimulation at high frequency (100 Hz) for 30 min increased the formation of labeled prostaglandin E2. Inclusion of glutathione also affected prostaglandin E2 formation. A lower concentration (0.1 mM) stimulated prostaglandin synthesis, while 1 mM glutathione was partially inhibitory. In both the rat and frog system, prostanoid synthesis was suppressed by indomethacin and aspirin.
利用大鼠和青蛙的匀浆及去鞘坐骨神经，研究了[1-14C]花生四烯酸向放射性标记前列腺素的转化。所研究的所有制剂均显示能合成前列腺素；所生成的特定前列腺素通过它们在三种不同溶剂系统的薄层色谱图上的迁移情况来表征。去鞘大鼠神经和匀浆均能合成前列腺素E2、前列腺素F2α、前列腺素D2、6-酮前列腺素F1α和血栓素B2。对于青蛙神经制剂，前列腺素E2是形成的主要前列腺素产物。有几种条件能够调节去鞘青蛙神经中前列腺素E2的产生。高频（100Hz）电刺激30分钟可增加标记前列腺素E2的形成。加入谷胱甘肽也会影响前列腺素E2的形成。较低浓度（0.1mM）刺激前列腺素合成，而1mM谷胱甘肽则有部分抑制作用。在大鼠和青蛙系统中，吲哚美辛和阿司匹林均能抑制前列腺素的合成。
