Wöhler Aliona, Gries Sabine K, Salzmann Rebekka J S, Krötz Christina, Wang Bingduo, Müller Paula, Klein Angelina, Schmidt-Wolf Ingo G H, Schaaf Sebastian, Schwab Robert, Lukacs-Kornek Veronika, Willms Arnulf G, Kornek Miroslaw T
Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany.
Department of Internal Medicine I University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University Bonn Germany.
J Extracell Biol. 2024 Sep 2;3(9):e70005. doi: 10.1002/jex2.70005. eCollection 2024 Sep.
Despite significant progress in the medical field, there is still a pressing need for minimal-invasive tools to assist with decision-making, especially in cases of polytrauma. Our team explored the potential of serum-derived large extracellular vesicles, so called microparticles/microvesicles/ectosomes, to serve as a supportive tool in decision-making in polytrauma situations. We focused on whether monocyte derived large EVs may differentiate between polytrauma patients with internal organ injury (ISS > 15) and those without. Thus, we compared our EV data to soluble biomarkers such as tumour necrosis factor alpha (TNF alpha) and Interleukin-8 (IL-8). From the blood of 25 healthy and 26 patients with polytrauma large EVs were isolated, purified, and characterized. TNF alpha and IL-8 levels were quantified. We found that levels of these monocyte derived large EVs were significantly higher in polytrauma patients with internal organ damage and correlated with the ISS. Interestingly, we also observed a decline in AnnVCD14 large EVs during normal recovery after trauma. Thus, inflammatory serological markers as TNF alpha and as IL-8 demonstrated an inability to discriminate between polytrauma patients with or without internal organ damage, such as spleen, kidney, or liver lacerations/ruptures. However, TNF and IL-8 levels were elevated in polytrauma cases overall when contrasted with healthy non-traumatic controls. These findings suggest that delving deeper into the potential of AnnV large EVs derived from monocytes could highly beneficial in the managment of polytrauma, potentially surpassing the efficacy of commonly used serum markers.
尽管医学领域取得了重大进展，但对于微创工具辅助决策仍有迫切需求，尤其是在多发伤的情况下。我们的团队探索了血清来源的大细胞外囊泡（即所谓的微粒/微囊泡/外泌体）在多发伤情况下作为决策辅助工具的潜力。我们关注单核细胞衍生的大细胞外囊泡是否可以区分有内脏损伤的多发伤患者（损伤严重度评分>15）和无内脏损伤的患者。因此，我们将我们的细胞外囊泡数据与可溶性生物标志物如肿瘤坏死因子α（TNFα）和白细胞介素-8（IL-8）进行了比较。从25名健康人和26名多发伤患者的血液中分离、纯化并鉴定了大细胞外囊泡。对TNFα和IL-8水平进行了定量。我们发现，这些单核细胞衍生的大细胞外囊泡水平在有内脏损伤的多发伤患者中显著更高，并且与损伤严重度评分相关。有趣的是，我们还观察到创伤后正常恢复过程中膜联蛋白V阳性CD14大细胞外囊泡数量下降。因此，TNFα和IL-8等炎性血清学标志物无法区分有无脾脏、肾脏或肝脏撕裂伤/破裂等内脏损伤的多发伤患者。然而，与健康非创伤对照组相比，多发伤病例总体上TNF和IL-8水平升高。这些发现表明，深入研究单核细胞衍生的膜联蛋白V大细胞外囊泡的潜力在多发伤管理中可能非常有益，可能超过常用血清标志物的功效。
