Mirara Francis, Dzidzienyo Daniel Kwadjo, Mwangi Maina
West Africa Centre for Crop Improvement (WACCI), University of Ghana, PMB 30 Legon, Accra, Ghana.
Biotechnology Centre, University of Ghana, P. O. Box 1195, Legon, Accra, Ghana.
Heliyon. 2024 Aug 10;10(16):e36141. doi: 10.1016/j.heliyon.2024.e36141. eCollection 2024 Aug 30.
Rice blast disease, caused by , poses a significant threat to global rice production, necessitating the development of effective and sustainable management strategies. Biological control using beneficial microbes like has emerged as a promising approach due to its ability to enhance plant resistance and reduce disease incidence. Nano-encapsulation of bacteria, which involves embedding beneficial microbes within nanomaterials, offers a novel method to improve the stability, survival, and efficacy of these biocontrol agents. This study evaluated the capacity of encapsulated D203, embedded within an alginate-bentonite coating infused with titanium nanoparticles (TNs), to stimulate defense responses in rice seedlings challenged by the the causal agent of rice blast disease. Encapsulation was achieved using the extrusion technique, with some modifications. Using a completely randomized design, the experiment was conducted in a greenhouse, with four treatments replicated four times. The experiment used the popular Kenyan rice variety "BASMATI 370". The study investigated the impact of strain D203 on the incidence, severity, and area under disease progress curves related to , as well as the expression of defense-related enzymes. The results demonstrated that rice plants derived from seeds coated with the D203 encapsulated strain exhibited higher levels of defense-related enzyme expression, including peroxidase (POD), phenylalanine ammonia-lyase (PAL), superoxide dismutase (SOD) and catalase (CAT), compared to controls. In addition, the incidence and severity of the disease were markedly lower in plants treated with encapsulated compared to controls, sometimes paralleling the efficacy of hexaconazole treatment. These findings suggest that the encapsulation of strain D203 has the potential to enhance resistance against rice blast disease by inducing systemic resistance through the production of antioxidant enzymes.
稻瘟病由[病原菌名称缺失]引起，对全球水稻生产构成重大威胁，因此需要制定有效且可持续的管理策略。利用[有益微生物名称缺失]等有益微生物进行生物防治，因其能够增强植物抗性并降低发病率，已成为一种有前景的方法。细菌的纳米包封，即将有益微生物包埋在纳米材料中，提供了一种提高这些生物防治剂稳定性、存活率和功效的新方法。本研究评估了包封在注入钛纳米颗粒（TNs）的海藻酸钠 - 膨润土涂层中的D203[细菌名称缺失]刺激受稻瘟病病原菌[病原菌名称缺失]挑战的水稻幼苗防御反应的能力。采用挤压技术并做了一些改进实现了包封。采用完全随机设计，在温室中进行实验，四个处理重复四次。实验使用了肯尼亚流行的水稻品种“BASMATI 370”。该研究调查了D203菌株对与[病原菌名称缺失]相关的发病率、严重程度和病害进展曲线下面积的影响，以及防御相关酶的表达。结果表明，与对照相比，用包封的D203[细菌名称缺失]菌株包衣种子培育的水稻植株表现出更高水平的防御相关酶表达，包括过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。此外，与对照相比，用包封的[细菌名称缺失]处理的植株病害的发病率和严重程度明显更低，有时与己唑醇处理的效果相当。这些发现表明，D203菌株的包封有可能通过产生抗氧化酶诱导系统抗性来增强对稻瘟病的抗性。
