Wang Saisai, Huang Yanna, Sun Yu, Wang Jinbin, Tang Xueming
School of Agriculture and Biology, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai, 200240, PR China.
Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Dongchuan Road 800, Minhang District, Shanghai, 200240, PR China.
Heliyon. 2024 Aug 8;10(16):e35966. doi: 10.1016/j.heliyon.2024.e35966. eCollection 2024 Aug 30.
Plant growth-promoting microorganisms (PGPMs), such as sp. YSD J2, promote plant development and stress resistance, while their role in flavonoids accumulation still needs to be further understood. To investigate the complex flavonoid biosynthesis pathway of L. var. sativus (tigernut), we compared sp. YSD J2 inoculation (YSD J2) and water inoculation (CK) groups. YSD J2 significantly elevated the content of indole-3-acetic acid (IAA) and orientin. Furthermore, when analyzing flavonoid metabolome, YSD J2 caused increased levels of uralenol, petunidin-3--glucoside-5--arabinoside, luteolin-7--glucuronide-(2 → 1)-glucuronide, kaempferol-3--neohesperidoside, cyanidin-3--(2″--glucosyl)glucoside, kaempferol-3--glucuronide-7--glucoside, quercetin-3--glucoside, luteolin-7--glucuronide-(2 → 1)-(2″-sinapoyl)glucuronide, and quercetin-4'--glucoside, which further enhanced antioxidant activity. We then performed RNA-seq and LC-MS/MS, aiming to validate key genes and related flavonoid metabolites under YSD J2 inoculation, and rebuild the gene-metabolites regulatory subnetworks. Furthermore, the expression patterns of the trans cinnamate 4-monooxygenase (), flavonol-3--L-rhamnoside-7--glucosyltransferase (), shikimate -hydroxycinnamoyltransferase (), chalcone isomerase (), flavonol synthase (), and anthocyanidin synthase () genes were confirmed by qRT-PCR. Additionally, 4 transcription factors (TF) (especially bHLH34, Cluster-37505.3) under YSD J2 inoculation are also engaged in regulating flavonoid accumulation. Moreover, the current work sheds new light on studying the regulatory effect of sp. YSD J2 on tigernut development and flavonoid biosynthesis.
植物促生微生物（PGPMs），如 sp. YSD J2，可促进植物生长发育并增强其抗逆性，但其在黄酮类化合物积累中的作用仍有待进一步研究。为探究虎坚果（L. var. sativus）复杂的黄酮类生物合成途径，我们比较了接种 sp. YSD J2（YSD J2）组和接种水（CK）组。YSD J2显著提高了吲哚-3-乙酸（IAA）和荭草素的含量。此外，在分析黄酮类代谢组时，YSD J2使乌拉尔醇、矮牵牛素-3--葡萄糖苷-5--阿拉伯糖苷、木犀草素-7--葡萄糖醛酸苷-(2→1)-葡萄糖醛酸苷、山柰酚-3--新橙皮糖苷、花青素-3--(2″--葡萄糖基)葡萄糖苷、山柰酚-3--葡萄糖醛酸苷-7--葡萄糖苷、槲皮素-3--葡萄糖苷、木犀草素-7--葡萄糖醛酸苷-(2→1)-(2″-芥子酰基)葡萄糖醛酸苷和槲皮素-4'--葡萄糖苷的水平升高，进一步增强了抗氧化活性。然后，我们进行了RNA测序和液相色谱-质谱联用（LC-MS/MS），旨在验证接种YSD J2条件下的关键基因和相关黄酮类代谢产物，并重建基因-代谢产物调控子网。此外，通过实时荧光定量PCR（qRT-PCR）证实了反式肉桂酸4-单加氧酶（）、黄酮醇-3--L-鼠李糖苷-7--葡萄糖基转移酶（）、莽草酸-羟基肉桂酰转移酶（）、查尔酮异构酶（）、黄酮醇合酶（）和花青素合酶（）基因的表达模式。此外，接种YSD J2条件下的4个转录因子（TF）（尤其是bHLH34，Cluster-37505.3）也参与调控黄酮类化合物的积累。此外，目前的工作为研究sp. YSD J2对虎坚果发育和黄酮类生物合成的调控作用提供了新的思路。
