Lindblom B, Andersson A
Biol Reprod. 1985 Apr;32(3):475-9. doi: 10.1095/biolreprod32.3.475.
Small muscle strips were dissected from the circular and longitudinal muscle layers of the human oviduct. The preparations showed rhythmic spontaneous activity when perfused by Krebs-Ringer buffer. Excitatory effects of the prostaglandin (PG) precursor arachidonic acid were totally blocked by the cyclooxygenase inhibitors 5,8,11,14-eicosatetraynoic acid (ETYA) and indomethacin. The latter drugs also caused a reversible inhibition of spontaneous activity in both muscle layers. After total inhibition produced by ETYA, the initial activity was restored by adding low concentrations of prostaglandin F2 alpha (PGF2 alpha) to the medium. PGE2 was able to reestablish the activity only in the longitudinal layer. It is concluded that isolated smooth muscle of the human oviduct has the capacity of generating PGs from both endogenous and exogenous substrate. The data also suggest that the formation of PGF2 alpha is a prerequisite for maintenance of normal tubal contractions.
从小肠输卵管的环形和纵形肌层中分离出小肌肉条。当用 Krebs-Ringer 缓冲液灌注时，这些标本显示出有节律的自发活动。前列腺素（PG）前体花生四烯酸的兴奋作用被环氧化酶抑制剂 5,8,11,14-二十碳四烯酸（ETYA）和吲哚美辛完全阻断。后一种药物也导致两层肌肉的自发活动出现可逆性抑制。在 ETYA 产生完全抑制后，通过向培养基中添加低浓度的前列腺素 F2α（PGF2α）可恢复初始活动。PGE2 仅能在纵形肌层中重新建立活动。结论是，人输卵管的分离平滑肌具有从内源性和外源性底物生成 PG 的能力。数据还表明，PGF2α 的形成是维持正常输卵管收缩的先决条件。
