College of Forestry, Northeast Forestry University, Harbin, China.
J Insect Sci. 2024 Jul 1;24(4). doi: 10.1093/jisesa/ieae087.
Poplar is a valuable tree species that is distributed all over the world. However, many insect pests infest poplar trees and have caused significant damage. To control poplar pests, we transformed a poplar species, Populus davidiana × P. bolleana Loucne, with the dsRNA of the chitinase gene of a poplar defoliator, Clostera anastomosis (Linnaeus) (Lepidoptera: Notodontidae), employing an Agrobaterium-mediated approach. The transgenic plant has been identified by cloning the T-DNA flanking sequences using TAIL-PCR and quantifying the expression of the dsRNA using qPCR. The toxicity assay of the transgenic poplar lines was carried out by feeding the target insect species (C. anastomosis). The results showed that, in C. anastomosis, the activity of chitinase was significantly decreased, consistent with the expression on mRNA levels, and the larval mortality was significantly increased. These results suggested that the transgenic poplar of dsRNA could be used for pest control.
杨树是一种分布广泛的有价值的树种。然而，许多害虫侵袭杨树，造成了严重的损害。为了控制杨树害虫，我们利用农杆菌介导的方法，将杨树品种 Populus davidiana × P. bolleana Loucne 转化为杨树食叶害虫 Clostera anastomosis (Linnaeus)（鳞翅目：枯叶蛾科）的几丁质酶基因的 dsRNA。利用 TAIL-PCR 克隆 T-DNA 侧翼序列，并通过 qPCR 定量 dsRNA 的表达，对转基因植物进行了鉴定。通过饲养目标昆虫物种（C. anastomosis）对转基因杨树品系进行了毒性测定。结果表明，在 C. anastomosis 中，几丁质酶的活性显著降低，与 mRNA 水平上的表达一致，幼虫死亡率显著增加。这些结果表明，dsRNA 的转基因杨树可用于害虫防治。
