Reyna-Campos Alma Ofelia, Ruiz-Villafan Beatriz, Macías-Rubalcava Martha Lydia, Langley Elizabeth, Rodríguez-Sanoja Romina, Sánchez Sergio
Departamento de Biología Molecular y Biotecnología del Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), 04510, CdMx, Mexico.
Posgrado en Ciencias Biológicas, Unidad de Posgrado, UNAM. , CdMx, 04510, Mexico.
AMB Express. 2024 Sep 3;14(1):97. doi: 10.1186/s13568-024-01761-w.
Lasso peptides, ribosomally synthesized and post-translationally modified peptides, are primarily produced by bacteria and some archaea. Streptomyces lasso peptides have been known for their antimicrobial, anticancer, and antiviral properties. However, understanding their role in the morphology and production of secondary metabolites remains limited. We identified a previously unknown lasso peptide gene cluster in the genome of Streptomyces sp. L06. This gene cluster (LASS) produces two distinct lasso peptides, morphosin-1 and - 2. Notably, morphosin-2 is a member of a new subfamily of lasso peptides, with BGCs exhibiting a similar structure. When LASS was expressed in different Streptomyces hosts, it led to exciting phenotypic changes, including the absence of spores and damage in aerial mycelium development. In one of the hosts, LASS even triggered antibiotic formation. These findings open up a world of possibilities, suggesting the potential role of morphosins in shaping Streptomyces' morphological and biochemical development.
套索肽是核糖体合成并经翻译后修饰的肽，主要由细菌和一些古生菌产生。链霉菌套索肽因其抗菌、抗癌和抗病毒特性而闻名。然而，对它们在次生代谢产物的形态和产生中所起作用的了解仍然有限。我们在链霉菌属菌株L06的基因组中鉴定出一个以前未知的套索肽基因簇。这个基因簇（LASS）产生两种不同的套索肽，即形态菌素-1和-2。值得注意的是，形态菌素-2是套索肽一个新亚家族的成员，其生物合成基因簇具有相似的结构。当LASS在不同的链霉菌宿主中表达时，会导致令人兴奋的表型变化，包括无孢子形成以及气生菌丝发育受损。在其中一个宿主中，LASS甚至引发了抗生素的形成。这些发现开启了一个充满可能性的世界，表明形态菌素在塑造链霉菌的形态和生化发育方面具有潜在作用。
