Wada S, Funae Y, Imaoka S, Kawamura M, Kinoshita Y, Sugimoto T, Nishio S, Kishimoto T, Maekawa M
Jpn J Cancer Res. 1985 Mar;76(3):192-6.
The concentration of N-butyl-N-(3-carboxypropyl)nitrosamine (BCPN), which is the major metabolite of the carcinogen N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN), was measured in the urine, thymus, liver, kidney, and bladder of rats orally administered with BBN. Since BCPN is a carboxylic acid, it forms an ester with 9-anthryldiazomethane (ADAM), which is a fluorescent labeling agent highly sensitive to carboxylic acids. Thus, BCPN and ADAM were reacted at 40 degrees for 1 hr, and the resulting ester was separated and measured by high-performance liquid chromatography (HPLC) with a reverse-phase type column. The range of measurement was 0 to 40 micrograms/ml, and the coefficient of variation (CV) was 3.8%. When 0.025% BBN was given orally to rats in tap water, the BCPN concentration in the urine was very high at 220 micrograms/ml, while it was 0.15 microgram/100 mg in the wet tissues of the thymus, 0.35 microgram/100 mg in the liver, 0.40 microgram/100 mg in the kidney, and 1.2 microgram/100 mg in the bladder. The BCPN concentration in the bladder, in which tumors are induced by the administration of BBN, was thus higher than those in the other organs.
给大鼠口服致癌物质N-丁基-N-(4-羟基丁基)亚硝胺（BBN）后，对其尿液、胸腺、肝脏、肾脏和膀胱中N-丁基-N-(3-羧丙基)亚硝胺（BCPN）的浓度进行了测量。BCPN是BBN的主要代谢产物，由于它是一种羧酸，可与对羧酸高度敏感的荧光标记剂9-蒽基重氮甲烷（ADAM）形成酯。因此，使BCPN与ADAM在40℃下反应1小时，然后通过使用反相柱的高效液相色谱法（HPLC）分离并测量生成的酯。测量范围为0至40微克/毫升，变异系数（CV）为3.8%。当用自来水给大鼠口服0.025%的BBN时，尿液中的BCPN浓度非常高，为220微克/毫升，而在胸腺湿组织中为0.15微克/100毫克，肝脏中为0.35微克/100毫克，肾脏中为0.40微克/100毫克，膀胱中为1.2微克/100毫克。因此，在经BBN给药可诱发肿瘤的膀胱中，BCPN浓度高于其他器官中的浓度。
