Guo Yuting, Xiao Ruiqi, Feng Jinxing, Wang Xiaoyun, Lai Jidong, Kang Wenqing, Li Yangfang, Zhu Xueping, Ji Tongzhen, Huang Xuerong, Pang Dan, An Yanbin, Meng Lihui, Wang Yajuan
Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Department of Neonatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
Heliyon. 2024 Aug 13;10(16):e35991. doi: 10.1016/j.heliyon.2024.e35991. eCollection 2024 Aug 30.
is the most common gram-negative pathogen to cause neonatal infections. Contemporary virulence characterization and antimicrobial resistance (AMR) data of neonatal isolates in China are limited.
A total of 159 strains isolated from neonates were collected and classified into invasive and non-invasive infection groups, according to their site of origin. The presence of virulence genes was determined using polymerase chain reaction (PCR). All the strains were subjected to antimicrobial susceptibility testing using the broth dilution method.
The top three virulence genes with the highest detection rates were (90.6 %), (88.7 %), and II (88.1 %). The prevalences of ( = 0.023), K1 ( = 0.019), ( < 0.001), and ( = 0.027) were significantly higher in the invasive infection group than in the non-invasive infection group. Resistance to ceftazixime, sulfamethoxazole/trimethoprim, and ciprofloxacin was 75.5 %, 65.4 %, and 48.4 %, respectively. Lower rates of resistance to ceftazidime ( = 0.022), cefepime ( = 0.005), ticarcillin/clavulanic acid ( = 0.020) and aztreonam ( = 0.001) were observed in the invasive infection group compared to the non-invasive infection group. The number of virulence genes carried by was positively correlated with the number of antibiotics to which the isolates were resistant ( = 0.71, = 0.016), and a specific virulence gene was associated with resistance to various species of antibiotics.
Neonatal isolates carried multiple virulence genes and were highly resistant to antibiotics. Further studies are needed to understand the molecular mechanisms underlying the association between virulence and AMR.
是引起新生儿感染最常见的革兰氏阴性病原体。中国新生儿分离株的当代毒力特征及抗菌药物耐药性（AMR）数据有限。
收集总共159株从新生儿分离出的菌株，根据其来源部位分为侵袭性和非侵袭性感染组。使用聚合酶链反应（PCR）测定毒力基因的存在情况。所有菌株采用肉汤稀释法进行抗菌药物敏感性试验。
检测率最高的前三个毒力基因分别是（90.6%）、（88.7%）和II（88.1%）。侵袭性感染组中（=0.023）、K1（=0.019）、（<0.001）和（=0.027）的流行率显著高于非侵袭性感染组。对头孢他啶、磺胺甲恶唑/甲氧苄啶和环丙沙星的耐药率分别为75.5%、65.4%和48.4%。与非侵袭性感染组相比，侵袭性感染组中对头孢唑肟（=0.022）、头孢吡肟（=0.005）、替卡西林/克拉维酸（=0.020）和氨曲南（=0.001）的耐药率较低。携带的毒力基因数量与分离株耐药的抗生素数量呈正相关（=0.71，=0.016），并且特定的毒力基因与对多种抗生素的耐药性相关。
新生儿分离株携带多种毒力基因且对抗生素高度耐药。需要进一步研究以了解毒力与AMR之间关联的分子机制。
