Feng Chengjie, Jia Hongbing, Yang Qian, Zou Qinghua
Department of Microbiology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
Department of Clinical Laboratory, China-Japan Friendship Hospital, Beijing 100029, China.
Microorganisms. 2025 Apr 21;13(4):954. doi: 10.3390/microorganisms13040954.
is a significant pathogen capable of inducing a variety of infections in both human and animal hosts. Despite its clinical significance, there is a lack of longitudinal research aimed at elucidating the genomic attributes that facilitate antimicrobial resistance and clonal dissemination in this bacterium. In this study, we investigated the genetic dynamics of antibiotic resistance and virulence factors within a collection of 137 isolates retrieved from a Chinese hospital over a 12-year period. Notably, a substantial increase in resistance to various antibiotics, including broad-spectrum β-lactams, aminoglycosides, and quinolones, was observed. Additionally, our study revealed the acquisition of diverse antibiotic resistance and virulence genes across different sequence types (STs). Among the STs, ST131 emerged as the most prevalent, exhibiting a high level of multidrug resistance. In contrast, ST73 and ST12 demonstrated a higher prevalence of virulence genes, suggestive of a potential trade-off between antibiotic resistance and virulence. What's more, we identified significant intra-clonal diversification and convergence of antibiotic resistance and virulence traits within the dominant ST131 group. These findings underscore the importance of longitudinal studies in understanding the evolution of bacterial pathogens and the necessity for ongoing research to inform public health strategies.
是一种重要的病原体，能够在人类和动物宿主中引发多种感染。尽管其具有临床意义，但缺乏旨在阐明促进该细菌产生抗菌耐药性和克隆传播的基因组特征的纵向研究。在本研究中，我们调查了从一家中国医院在12年期间收集的137株分离株中抗生素耐药性和毒力因子的遗传动态。值得注意的是，观察到对包括广谱β-内酰胺类、氨基糖苷类和喹诺酮类在内的各种抗生素的耐药性大幅增加。此外，我们的研究揭示了不同序列类型（STs）中多种抗生素耐药性和毒力基因的获得情况。在这些STs中，ST131最为普遍，表现出高水平的多重耐药性。相比之下，ST73和ST12的毒力基因患病率较高，这表明抗生素耐药性和毒力之间可能存在权衡。此外，我们在占主导地位的ST131组中发现了抗生素耐药性和毒力特征的显著克隆内多样化和趋同现象。这些发现强调了纵向研究在理解细菌病原体进化中的重要性，以及持续开展研究以为公共卫生策略提供信息的必要性。
