1Orbeli Institute of Physiology NAS RA, 0028, Yerevan, Armenia.
4Yerevan State Medical University after Mkhitar Heratsi, 0025, Armenia.
Georgian Med News. 2024 Jun(351):116-124.
Thermal burns are the most common type of burn injuries. Medical treatment for burns is crucial, especially for third-degree burns and when a significant surface area of the body is affected. One of the most pressing issues in modern medicine is the search for new effective means to accelerate the healing of burn wounds. Oxygen radicals play a significant role in maintaining homeostasis, forming the body's resistance to infection, and ensuring the regeneration of organs and tissues. In this study, a superoxide (O2-)-producing enzyme (SPE) from raspberries was applied (topically to the skin, injected under the wound surface, with solution concentrations of 12.75% and 5%) after a third-degree thermal burn to determine its reparative effects on the skin. To assess the condition of the animals that had suffered burn injuries and the healing process, blood parameters were analyzed, and cytogenetic indices of bone marrow from the femur of the animals were studied: mitotic index, number of polyploid cells, and chromosomal aberrations. When analyzing hematological, cytogenetic, and histological parameters, significant differences were found between the «clean burn» groups and the groups in which SPE was used in different concentrations and methods of application. The use of SPE in both concentrations contributed to a reduction in the area of burn wounds compared to a «clean burn». The survival rate of animals for 30 days (before the end of the experiment) was 100% when using a 12.75% SPE solution and 50% when using a 5% SPE solution. The use of SPE led to significant differences in hematological parameters from the «clean burn» group throughout the entire duration of the experiment, showing a tendency to normalize the parameters. Under the influence of the 12.75% SPE solution, there was a tendency toward normalization of the mitotic index, along with a significant reduction in the percentage of polyploid cells and chromosomal aberrations, which may indicate its beneficial effects. This study found that a 12.75% SPE solution derived from raspberries was more effective and had healing properties on third-degree thermal burns, promoting rapid healing of the burn wound.
热力烧伤是最常见的烧伤类型。烧伤的医疗治疗至关重要，特别是对于三度烧伤和身体大面积受影响的情况。现代医学中最紧迫的问题之一是寻找新的有效手段来加速烧伤伤口的愈合。氧自由基在维持体内平衡、形成身体抗感染能力以及确保器官和组织再生方面发挥着重要作用。在这项研究中，应用了一种来自覆盆子的超氧化物（O2-）产生酶（SPE）（局部涂于皮肤，在伤口表面下注射，溶液浓度为 12.75%和 5%），以确定其对皮肤的修复作用三度热烧伤后。为了评估遭受烧伤的动物的状况和愈合过程，分析了血液参数，并研究了动物股骨骨髓的细胞遗传学指数：有丝分裂指数、多倍体细胞数和染色体畸变。在分析血液学、细胞遗传学和组织学参数时，在“清洁烧伤”组和以不同浓度和应用方法使用 SPE 的组之间发现了显著差异。与“清洁烧伤”相比，使用两种浓度的 SPE 均有助于减少烧伤面积。使用 12.75% SPE 溶液时，动物的 30 天存活率（在实验结束前）为 100%，使用 5% SPE 溶液时为 50%。使用 SPE 导致整个实验过程中血液参数与“清洁烧伤”组有显著差异，表现出参数正常化的趋势。在 12.75% SPE 溶液的影响下，有丝分裂指数有正常化的趋势，同时多倍体细胞和染色体畸变的百分比显著降低，这可能表明其有益作用。本研究发现，来自覆盆子的 12.75% SPE 溶液对三度热力烧伤更有效，具有愈合特性，可促进烧伤伤口的快速愈合。
