Department of Genetics and Plant Breeding, CCS University, Meerut, Uttar Pradesh, 250004, India.
Sci Rep. 2020 Sep 3;10(1):14624. doi: 10.1038/s41598-020-71526-5.
SET domain genes (SDGs) that are involved in histone methylation have been examined in many plant species, but have never been examined in bread wheat; the histone methylation caused due to SDGs is associated with regulation of gene expression at the transcription level. We identified a total of 166 bread wheat TaSDGs, which carry some interesting features including the occurrence of tandem/interspersed duplications, SSRs (simple sequence repeats), transposable elements, lncRNAs and targets for miRNAs along their lengths and transcription factor binding sites (TFBS) in the promoter regions. Only 130 TaSDGs encoded proteins with complete SET domain, the remaining 36 proteins had truncated SET domain. The TaSDG encoded proteins were classified into six classes (I-V and VII). In silico expression analysis indicated relatively higher expression (FPKM > 20) of eight of the 130 TaSDGs in different tissues, and downregulation of 30 TaSDGs under heat and drought at the seedling stage. qRT-PCR was also conducted to validate the expression of seven genes at the seedling stage in pairs of contrasting genotypes in response to abiotic stresses (water and heat) and biotic stress (leaf rust). These genes were generally downregulated in response to the three stresses examined.
已在许多植物物种中研究了参与组蛋白甲基化的 SET 结构域基因（SDG），但从未在面包小麦中进行过研究；由于 SDG 引起的组蛋白甲基化与转录水平的基因表达调控有关。我们总共鉴定了 166 个小麦 TaSDG，它们具有一些有趣的特征，包括串联/散布重复、SSR（简单序列重复）、转座元件、lncRNA 和 miRNA 靶标在其长度上以及启动子区域中的转录因子结合位点（TFBS）。只有 130 个 TaSDG 编码具有完整 SET 结构域的蛋白质，其余 36 个蛋白质具有截断的 SET 结构域。TaSDG 编码的蛋白质被分为六类（I-V 和 VII）。计算机表达分析表明，在不同组织中，130 个 TaSDG 中的 8 个的相对较高表达（FPKM>20），并且在幼苗期热和干旱下 30 个 TaSDG 的下调。还进行了 qRT-PCR 以验证在幼苗期在一对对照基因型之间对非生物胁迫（水和热）和生物胁迫（叶锈病）的七个基因的表达。这些基因通常在三种受检压力下均下调。
