College of Ecology and Environment, Nanjing Forestry University, Nanjing, China.
Department of Environmental Biotechnology, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), Daejeon, Republic of Korea.
BMC Microbiol. 2024 Sep 4;24(1):321. doi: 10.1186/s12866-024-03477-0.
With the development of industry and modern manufacturing, nondegradable low-density polyethylene (LDPE) has been widely used, posing a rising environmental hazard to natural ecosystems and public health. In this study, we isolated a series of LDPE-degrading fungi from landfill sites and carried out LDPE degradation experiments by combining highly efficient degrading fungi in pairs. The results showed that the mixed microorganisms composed of Alternaria sp. CPEF-1 and Trametes sp. PE2F-4 (H-3 group) had a greater degradation effect on heat-treated LDPE (T-LDPE). After 30 days of inoculation with combination strain H-3, the weight loss rate of the T-LDPE film was approximately 154% higher than that of the untreated LDPE (U-LDPE) film, and the weight loss rate reached 0.66 ± 0.06%. Environmental scanning electron microscopy (ESEM) and Fourier transform infrared spectroscopy (FTIR) were used to further investigate the biodegradation impacts of T-LDPE, including the changes on the surface and depolymerization of the LDPE films during the fungal degradation process. Our findings revealed that the combined fungal treatment is more effective at degrading T-LDPE than the single strain treatment, and it is expected that properly altering the composition of the microbial community can help lessen the detrimental impact of plastics on the environment.
随着工业和现代制造业的发展，不可降解的低密度聚乙烯（LDPE）得到了广泛的应用，对自然生态系统和公众健康造成了越来越大的环境危害。在本研究中，我们从垃圾填埋场中分离出一系列 LDPE 降解真菌，并通过组合高效降解真菌对 LDPE 进行降解实验。结果表明，由链格孢菌 CPEF-1 和粗毛栓菌 PE2F-4 组成的混合微生物（H-3 组）对热处理 LDPE（T-LDPE）具有更大的降解效果。在接种组合菌株 H-3 30 天后，T-LDPE 薄膜的失重率比未处理的 LDPE（U-LDPE）薄膜高约 154%，失重率达到 0.66±0.06%。环境扫描电子显微镜（ESEM）和傅里叶变换红外光谱（FTIR）进一步研究了 T-LDPE 的生物降解影响，包括真菌降解过程中 LDPE 薄膜表面的变化和聚合物的解聚。我们的研究结果表明，与单一菌株处理相比，联合真菌处理更有效地降解 T-LDPE，预计适当改变微生物群落的组成可以帮助减轻塑料对环境的不利影响。
