Aquaculture Research Lab, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei, China.
Ecotoxicol Environ Saf. 2021 Aug;219:112347. doi: 10.1016/j.ecoenv.2021.112347. Epub 2021 May 25.
Cherax quadricarinatus is a type of large freshwater crayfish that is characterized by rapid growth and formidable adaptability. It has also been widely cultured and studied as a model organism. Aeromonas veronii is the dominant pathogen in aquatic environments and the primary threat to aquaculture's economic stability. To better understand the interactions between C. quadricarinatus and A. veronii, high-throughput RNA sequencing of the C. quadricarinatus hepatopancreas was carried out on a control group, susceptible group (6 h after infection), and resistant group (48 h after infection). A total of 65,850,929 genes were obtained. Compared with the control group, 2616 genes were up-regulated and 1551 genes were down-regulated in the susceptible group; while 1488 genes were up-regulated and 1712 genes were down-regulated in the resistant group. GO and KEGG analysis showed that these differentially expressed genes (DEGs) were associated with multiple immune pathways, including Toll-like receptors (TLRs), antigen processing and presentation, NOD-like receptor signaling pathway, phagosome, lysosome, JAK-STAT signaling pathway. qRT-PCR showed that infection by A. veronii changed the expression pattern of the serine proteinase inhibitor (SPI), crustacean hyperglycemic hormone (CHH), anti-lipopolysaccharide factor (ALF), and extracellular copper/zinc superoxide dismutase (SOD1), all of which were significantly higher than in the control group up to 48 h after infection. In addition, detection of superoxide dismutase (SOD), catalase (CAT), lysozyme (LZM), and phenoloxidase (PO) activity, as well as ceruloplasmin (CP) concentration at different times after infection showed diverse trends. Furthermore, pathological sections obtained 24 h after infection show lesions on the hepatopancreas and intestinal tissues caused by A. veronii. The results of this study provide a foundation for analyzing the immune mechanism of C. quadricarinatus infected with A. veronii at the transcriptional level and a theoretical basis for screening disease-resistant individuals to ensure healthy economic development of the aquatic industry.
拟穴青蟹是一种生长迅速、适应性强的大型淡水小龙虾，已被广泛养殖和研究为模式生物。维氏气单胞菌是水生环境中的主要病原体，也是水产养殖经济稳定的主要威胁。为了更好地了解拟穴青蟹和维氏气单胞菌之间的相互作用，对对照组、易感组（感染后 6 小时）和抗性组（感染后 48 小时）的拟穴青蟹肝胰腺进行了高通量 RNA 测序。共获得 65850929 个基因。与对照组相比，易感组中有 2616 个基因上调，1551 个基因下调；而抗性组中有 1488 个基因上调，1712 个基因下调。GO 和 KEGG 分析表明，这些差异表达基因（DEGs）与多种免疫途径有关，包括 Toll 样受体（TLRs）、抗原加工和呈递、NOD 样受体信号通路、吞噬体、溶酶体、JAK-STAT 信号通路。qRT-PCR 显示，维氏气单胞菌感染改变了丝氨酸蛋白酶抑制剂（SPI）、甲壳动物高血糖激素（CHH）、抗脂多糖因子（ALF）和细胞外铜/锌超氧化物歧化酶（SOD1）的表达模式，感染后 48 小时内，其表达均明显高于对照组。此外，在感染后不同时间检测超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、溶菌酶（LZM）和酚氧化酶（PO）活性以及铜蓝蛋白（CP）浓度，结果显示出不同的趋势。此外，感染后 24 小时获得的病理切片显示维氏气单胞菌引起的肝胰腺和肠道组织病变。本研究为分析拟穴青蟹转录水平感染维氏气单胞菌的免疫机制提供了基础，为筛选抗病个体提供了理论依据，以确保水产业的健康经济发展。
