枯草芽孢杆菌168富马酸酶基因（citG）的完整核苷酸序列
Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.
作者信息
Miles J S, Guest J R
出版信息
Nucleic Acids Res. 1985 Jan 11;13(1):131-40. doi: 10.1093/nar/13.1.131.
Abstract
The nucleotide sequence of a 2.14 kb fragment of Bacillus subtilis DNA containing the citG gene encoding fumarase was determined using the dideoxy chain termination method. The citG coding region of 1392 base pairs (464 codons) was identified, and the deduced Mr (50425) is in good agreement with that of the protein identified from expression in Escherichia coli maxicells. There is no sequence homology between the B. subtilis and E. coli fumarases. Overlapping potential promoter sequences have been identified for sigma 28, sigma 37 and sigma 55 RNA polymerase holoenzymes. The DNA fragment also contains the proximal part of the gerA locus, responsible for L-alanine-sensitive spore germination.
摘要
采用双脱氧链终止法测定了枯草芽孢杆菌DNA中一个包含编码延胡索酸酶的citG基因的2.14 kb片段的核苷酸序列。鉴定出了1392个碱基对（464个密码子）的citG编码区，推导的Mr（50425）与在大肠杆菌大细胞中表达鉴定出的蛋白质的Mr高度一致。枯草芽孢杆菌和大肠杆菌的延胡索酸酶之间没有序列同源性。已鉴定出与σ28、σ37和σ55 RNA聚合酶全酶重叠的潜在启动子序列。该DNA片段还包含gerA基因座的近端部分，负责L-丙氨酸敏感的孢子萌发。
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