大肠杆菌延胡索酸酶基因fumA的完整核苷酸序列。
Complete nucleotide sequence of the fumarase gene fumA, of Escherichia coli.
作者信息
Miles J S, Guest J R
出版信息
Nucleic Acids Res. 1984 Apr 25;12(8):3631-42. doi: 10.1093/nar/12.8.3631.
Abstract
The nucleotide sequence of a 2.41 kb fragment of E. coli DNA containing the fumA gene encoding fumarase was determined using the dideoxy chain termination method. The initiation and termination sites of fumA transcription were located using RNA:DNA hybridisation with single-stranded M13 probes. The length of the fumA transcript was estimated as 1760 +/- 15 nucleotides and the fumA coding region of 1647 base pairs (549 codons) was identified. The deduced molecular weight of the fumarase protein (Mr = 60163) is in good agreement with that of the protein identified by the maxicell procedure. The DNA fragment also contains the 5' end of the manA gene encoding mannose 6-phosphate isomerase, which is transcribed with the opposite polarity to fumA .
摘要
利用双脱氧链终止法测定了大肠杆菌DNA中一个2.41kb片段的核苷酸序列，该片段含有编码延胡索酸酶的fumA基因。采用RNA:DNA与单链M13探针杂交的方法确定了fumA转录的起始和终止位点。fumA转录本的长度估计为1760±15个核苷酸，并鉴定出了1647个碱基对（549个密码子）的fumA编码区。推导的延胡索酸酶蛋白分子量（Mr = 60163）与通过大细胞程序鉴定的蛋白分子量高度一致。该DNA片段还包含编码甘露糖6-磷酸异构酶的manA基因的5'端，其转录方向与fumA相反。
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