Hum O S, Ghista D N, Brash J L
Thromb Res. 1985 Apr 15;38(2):163-72. doi: 10.1016/0049-3848(85)90058-1.
Contact angle measurements, histological examinations and platelet adhesion tests on native and glutaraldehyde-treated porcine aortic valve leaflets, are reported. Contact angles measured with water or saline on native and treated leaflets do not differ appreciably. Values of less than 10 degrees for both the dry and wet leaflets were observed. Histological examination of the native and fixed tissue surfaces was carried out with both optical and scanning electron microscopy. Native leaflet surfaces appeared to be smoother with longer and straighter fibres beneath the surface than treated leaflets. Platelet adhesion tests were performed with fresh, non-anticoagulated human whole blood as well as with platelet suspension. Static platelet adhesion tests were carried out by depositing droplets of blood on the test surface. The blood droplets were washed off after a contact time of 2 minutes for whole blood and up to 32 minutes for platelet suspension. The adhering platelets were microscopically observed and counted. The platelet density on the native leaflets was found to be significantly higher than on the treated tissues. However, the platelet density did not vary with glutaraldehyde concentration in the range from 0.2 to 1.0% (v/v).
本文报道了对天然猪主动脉瓣叶和经戊二醛处理的猪主动脉瓣叶进行的接触角测量、组织学检查和血小板黏附试验。用蒸馏水或生理盐水测量的天然瓣叶和处理后瓣叶的接触角没有明显差异。观察到干瓣叶和湿瓣叶的接触角值均小于10度。用光学显微镜和扫描电子显微镜对天然组织表面和固定组织表面进行了组织学检查。天然瓣叶表面似乎比处理后的瓣叶更光滑，其表面下的纤维更长、更直。用新鲜的、未抗凝的人体全血以及血小板悬液进行了血小板黏附试验。静态血小板黏附试验是通过将血滴沉积在测试表面上进行的。全血接触2分钟后、血小板悬液接触长达32分钟后，将血滴冲洗掉。对黏附的血小板进行显微镜观察和计数。发现天然瓣叶上的血小板密度显著高于处理后的组织。然而，在0.2%至1.0%（v/v）的戊二醛浓度范围内，血小板密度并未随其浓度变化。
