School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China; State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing, 210023, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510275, China.
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510275, China.
Water Res. 2024 Nov 15;266:122356. doi: 10.1016/j.watres.2024.122356. Epub 2024 Aug 29.
Research on the microbial community and function of the anammox process for environmentally friendly wastewater treatment has achieved certain success, which may mean more universal insights are needed. However, the comprehensive understanding of the anammox process is constrained by the limited taxonomic assignment and functional characterization of anammox microbiota, primarily due to the scarcity of high-quality genomes for most organisms. This study reported a global genome catalog of anammox microbiotas based on numerous metagenomes obtained from both lab- and full-scale systems. A total of 1376 candidate species from 7474 metagenome-assembled genomes were used to construct the genome catalog, providing extensive microbial coverage (averaged of 92.40 %) of anammox microbiota. Moreover, a total of 64 core genera and 44 core species were identified, accounting for approximately 64.25 % and 43.97 %, respectively, of anammox microbiota. The strict core genera encompassed not only functional bacteria (e.g., Brocadia, Desulfobacillus, Zeimonas, and Nitrosomonas) but also two candidate genera (UBA12294 and OLB14) affiliated with the order Anaerolineales. In particular, core denitrifying bacteria with observably taxonomic diversity exhibited diverse functional profiles; for instance, the potential of carbohydrate metabolism in Desulfobacillus and Zeimonas likely improves the mixotrophic lifestyle of anammox microbiota. Besides, a noteworthy association was detected between anammox microbiota and system type. Microbiota in coupling system exhibited complex diversity and interspecies interactions by limiting numerous core denitrifying bacteria. In summary, the constructed catalog substantially expands our understanding of the core community and their functions of anammox microbiota, providing a valuable resource for future studies on anammox systems.
基于实验室和实际规模系统中获得的大量宏基因组，本研究报道了一个氨氧化菌微生物组的全基因组目录。该目录共包含 1376 个候选种，来自 7474 个宏基因组组装基因组，为氨氧化菌微生物组提供了广泛的微生物覆盖度（平均为 92.40%）。此外，共鉴定出 64 个核心属和 44 个核心种，分别占氨氧化菌微生物组的约 64.25%和 43.97%。严格核心属不仅包括功能细菌（如 Brocadia、Desulfobacillus、Zeimonas 和 Nitrosomonas），还包括两个与 Anaerolineales 目相关的候选属（UBA12294 和 OLB14）。特别是，具有明显分类多样性的核心脱氮菌表现出多样化的功能特征；例如，Desulfobacillus 和 Zeimonas 中碳水化合物代谢的潜力可能改善了氨氧化菌微生物组的混合营养生活方式。此外，还检测到氨氧化菌微生物组与系统类型之间存在显著关联。偶联系统中的微生物组通过限制大量核心脱氮菌表现出复杂的多样性和种间相互作用。总之，构建的目录大大扩展了我们对氨氧化菌微生物组核心群落及其功能的理解，为未来的氨氧化系统研究提供了有价值的资源。
