Zulkarnaini Zulkarnaini, Komala Puti Sri, Putra Randi Permana, Ali Muhammad, Matsuura Norihisa, Koike Kazuyoshi, Wang Jinsong, Mawarda Panji Cahya, Yamamoto-Ikemoto Ryoko
Department of Environmental Engineering, Universitas Andalas, Padang 25175, Indonesia E-mail:
Department of Environmental Engineering, Universitas Andalas, Padang 25175, Indonesia.
Water Sci Technol. 2025 Apr;91(7):811-826. doi: 10.2166/wst.2025.050. Epub 2025 Apr 4.
This study presents a novel strategy for cultivating anammox bacteria from tropical environments using a filter bioreactor (FtBR). Two bioreactors were inoculated with sediment sludge from an Indonesian lake and operated at different temperatures: tropical ambient (22-28 °C) in Reactor 1 and 35 °C in Reactor 2. After 106 days, Reactor 1 developed a red carmine anammox biofilm, while Reactor 2 remained similar to its initial state. Reactor 1 achieved a higher and more stable nitrogen removal rate (0.27 kg-N/m·d) compared with Reactor 2 (0.21 kg-N/m·d), indicating a 28.6% greater efficiency. The operational temperature significantly influenced the diversity and abundance of anammox bacteria. (6.20%) was detected in Reactor 1, whereas (7.64%) and (1.77%) were found only in Reactor 2. Additionally, was more abundant in Reactor 1 (20.04%) than in Reactor 2 (6.84%). These findings demonstrate that temperature plays a crucial role in starting the anammox process in FtBRs with a resident inoculum from tropical environments, significantly affecting bacterial growth and nitrogen removal efficiency.
本研究提出了一种利用过滤生物反应器（FtBR）从热带环境中培养厌氧氨氧化细菌的新策略。两个生物反应器接种了来自印度尼西亚一个湖泊的沉积物污泥，并在不同温度下运行：反应器1为热带环境温度（22 - 28°C），反应器2为35°C。106天后，反应器1形成了红色的厌氧氨氧化生物膜，而反应器2仍与初始状态相似。与反应器2（0.21 kg-N/m·d）相比，反应器1实现了更高且更稳定的氮去除率（0.27 kg-N/m·d），效率高出28.6%。运行温度显著影响了厌氧氨氧化细菌的多样性和丰度。在反应器1中检测到了（6.20%），而（7.64%）和（1.77%）仅在反应器2中发现。此外，在反应器1中（20.04%）比在反应器2中（6.84%）更为丰富。这些发现表明，温度在以热带环境中的常驻接种物启动FtBR中的厌氧氨氧化过程中起着关键作用，显著影响细菌生长和氮去除效率。
