Mazurová J
Vet Med (Praha). 1985 Apr;30(4):247-54.
The method of rapid slide agglutination and coagglutination was tested in the detection of Haemophilus equigenitalis (Taylorella equigenitalis)--the causal agent of contagious equine metritis (CEM). It was demonstrated that both methods were suitable for the serological diagnosis of the species under study. The antisera obtained from rabbits immunized with Haemophilus equigenitalis strains treated in different ways were specific, but with different antibody titres. When cross reactions with other species of microorganisms were verified, the antisera did not react with any of the strains, even after binding them to protein A of the positive strain Staphylococcus aureus--Cowan I. Coagglutination was much more rapid and pronounced than the ordinary rapid agglutination test. It was characterized by a low consumption of specific antiserum. The specific antibodies bound to staphylococci were kept at the temperature of 4 degrees C for several months without losing agglutinin activity.
采用快速玻片凝集法和协同凝集法检测马生殖嗜血杆菌（马泰勒菌）——马传染性子宫炎（CEM）的病原体。结果表明，这两种方法均适用于所研究菌种的血清学诊断。用不同方法处理的马生殖嗜血杆菌菌株免疫家兔获得的抗血清具有特异性，但抗体效价不同。在验证与其他微生物菌种的交叉反应时，即使将抗血清与阳性菌株金黄色葡萄球菌 Cowan I 的蛋白 A 结合后，抗血清也不与任何菌株发生反应。协同凝集比普通快速凝集试验更为迅速和明显。其特点是特异性抗血清消耗低。与葡萄球菌结合的特异性抗体在4℃下保存数月，凝集素活性不丧失。
