Tainturier D J, Delmas C F, Dabernat H J
J Clin Microbiol. 1981 Oct;14(4):355-60. doi: 10.1128/jcm.14.4.355-360.1981.
Seventeen strains of haemophilus equigenitalis isolated from the cervix, clitoris, and urethra of mares were biochemically characterized with the API 10E and APIZYM test kit systems, conventional biochemical tests, and the porphyrin test. Antisera were prepared in rabbits. All of the strains were positive to the porphyrin test, and the requirement for factor X (hemin) or V (nicotinamide adenine dinucleotide) was not shown. Catalase, oxidase, phosphatase, and phosphoamidase tests were positive with all of the strains. Aminopeptidase (arylamidase) activity has been detected on beta-naphthylamide derivatives of eight amino acids and of di- and tripeptides. No glycosidase activity was found. Antisera prepared in rabbits strongly agglutinated all H. equigenitalis strains, but none of the various other bacterial strains. These characteristics should prove to be useful in the identification of H. equigenitalis.
从母马子宫颈、阴蒂和尿道分离出的17株马生殖嗜血杆菌，采用API 10E和APIZYM测试试剂盒系统、传统生化测试以及卟啉测试进行生物化学特征鉴定。用兔子制备抗血清。所有菌株的卟啉测试均呈阳性，未显示对因子X（血红素）或V（烟酰胺腺嘌呤二核苷酸）的需求。所有菌株的过氧化氢酶、氧化酶、磷酸酶和磷酸酰胺酶测试均呈阳性。已检测到对8种氨基酸以及二肽和三肽的β-萘酰胺衍生物的氨肽酶（芳基酰胺酶）活性。未发现糖苷酶活性。用兔子制备的抗血清能强烈凝集所有马生殖嗜血杆菌菌株，但不能凝集其他各种细菌菌株。这些特征应有助于马生殖嗜血杆菌的鉴定。
