Papp Sara B, Hogins Jacob, Mekala Shreya, Christie Alana, Chavez Juliann, Reitzer Larry, Zimmern Philippe E
UT Southwestern Medical Center, Dallas, TX.
The University of Texas at Dallas, Richardson, TX.
Urology. 2024 Dec;194:82-88. doi: 10.1016/j.urology.2024.08.066. Epub 2024 Sep 3.
To evaluate bacterial growth in the bladder as a major virulence factor during urinary tract infections (UTIs), we assessed the variability of uropathogenic bacterial growth in urine samples over multiple timepoints from 3 postmenopausal women with no history of UTI.
Following IRB approval, postmenopausal women who never had a UTI provided mid-stream urine samples 3 times daily (8 a.m., 12 p.m., and 4 p.m.), twice a week over 2 weeks, as well as dietary intake logs. Each sample was studied for pH and bacterial growth using 3 uropathogenic Escherichia coli (E.coli) strains (LRPF007, KE40, UTI89), 1 uropathogenic Enterococcus faecalis strain (HRH40), and 1 non-pathogenic E. coli strain (W3110).
Similar mean growth yields were observed for the uropathogenic strains (mean ∆OD =0.1-0.13) with high variability (standard deviation [SD]= 0.12-0.28) and lower mean growth for the non-pathogenic strain (mean ∆OD =0.05). Urine from each individual at different collection times never had the same bacterial growth potential for any bacterial strain. There were significant associations between urine pH decrease with higher fat (P = .017), vitamin D (P = .02), magnesium (P = .049), fluid (P = .013), and ash (P = .01) intake.
Major variations were observed in the bacterial growth response in the urine of the same individuals. These variations imply a requirement for a normalization procedure, eg, growth in synthetic urine, for analysis of bacterial growth in urine. There were significant associations between decreased urine pH and several nutrients found in foods that are classified as highly acidic.
为评估膀胱中的细菌生长作为尿路感染（UTI）期间的一种主要毒力因子，我们在来自3名无UTI病史的绝经后女性的尿液样本中，在多个时间点评估了尿路致病性细菌生长的变异性。
经机构审查委员会（IRB）批准后，从未患过UTI的绝经后女性每天提供3次（上午8点、中午12点和下午4点）的中段尿液样本，在2周内每周两次，同时提供饮食摄入记录。使用3种尿路致病性大肠杆菌（E.coli）菌株（LRPF007、KE40、UTI89）、1种尿路致病性粪肠球菌菌株（HRH40）和1种非致病性大肠杆菌菌株（W3110）对每个样本进行pH值和细菌生长研究。
观察到尿路致病性菌株的平均生长产量相似（平均吸光度变化[∆OD]=0.1 - 0.13），变异性高（标准差[SD]=0.12 - 0.28），而非致病性菌株的平均生长较低（平均∆OD =0.05）。在不同采集时间，每个个体的尿液对于任何细菌菌株都从未具有相同的细菌生长潜力。尿液pH值降低与较高的脂肪（P = 0.017）、维生素D（P = 0.02）、镁（P = 0.049）、液体（P = 0.013）和灰分（P = 0.01）摄入量之间存在显著关联。
在同一个体的尿液中观察到细菌生长反应存在主要差异。这些差异意味着在分析尿液中的细菌生长时需要一种标准化程序，例如在合成尿液中生长。尿液pH值降低与食物中归类为高酸性的几种营养素之间存在显著关联。
