Department of Orthopedics, First Hospital of China Medical University, No. 155 Nanjing North Street, Shenyang, Liaoning Province, China.
Department of Medical Oncology, First Hospital of China Medical University, No. 155 Nanjing North Street, Shenyang, China.
J Nanobiotechnology. 2024 Sep 5;22(1):540. doi: 10.1186/s12951-024-02757-1.
To assess the efficacy of a novel 3D biomimetic hydrogel scaffold with immunomodulatory properties in promoting fracture healing. Immunomodulatory scaffolds were used in cell experiments, osteotomy mice treatment, and single-cell transcriptomic sequencing. In vitro, fluorescence tracing examined macrophage mitochondrial transfer and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Scaffold efficacy was assessed through alkaline phosphatase (ALP), Alizarin Red S (ARS) staining, and in vivo experiments. The scaffold demonstrated excellent biocompatibility and antioxidant-immune regulation. Single-cell sequencing revealed a shift in macrophage distribution towards the M2 phenotype. In vitro experiments showed that macrophage mitochondria promoted BMSCs' osteogenic differentiation. In vivo experiments confirmed accelerated fracture healing. The GAD/Ag-pIO scaffold enhances osteogenic differentiation and fracture healing through immunomodulation and promotion of macrophage mitochondrial transfer.
评估具有免疫调节特性的新型 3D 仿生水凝胶支架在促进骨折愈合方面的功效。免疫调节支架在细胞实验、骨切开术小鼠治疗和单细胞转录组测序中得到了应用。在体外，荧光示踪法检测了巨噬细胞线粒体转移和骨髓间充质干细胞（BMSCs）的成骨分化。通过碱性磷酸酶（ALP）、茜素红 S（ARS）染色和体内实验评估支架的功效。该支架表现出良好的生物相容性和抗氧化-免疫调节作用。单细胞测序显示巨噬细胞分布向 M2 表型转变。体外实验表明，巨噬细胞线粒体促进了 BMSCs 的成骨分化。体内实验证实了骨折愈合的加速。GAD/Ag-pIO 支架通过免疫调节和促进巨噬细胞线粒体转移来增强成骨分化和骨折愈合。
