Liu Rundong, Paguirigan Jaycee Augusto, Hur Jae-Seoun, Kim Wonyong
a Korean Lichen Research Institute, Sunchon National University.
b Department of Biological Sciences, College of Science, University of Santo Tomas.
Mycoscience. 2024 Jan 31;65(1):19-27. doi: 10.47371/mycosci.2023.11.002. eCollection 2024.
An endophytic fungus, sp. NG-25, produces a set of structurally related polyketides including cercosporamide, phomodione, and usnic acid, among which, cercosporamide has been reported to have strong antifungal and anticancer activities. In this study, sp. NG-25 was grown in seven growth media to determine the optimal culture condition conducive for cercosporamide production. Cercosporamide production peaked on the eighteenth day of incubation in beef peptone dextrose (BPD) broth media. The cercosporamide titer reached to an average of 77.5 µg/mL in BPD. Paper disk diffusion assay revealed that culture filtrate containing cercosporamide as a major constituent inhibited the growth of taxonomically diverse plant pathogens, including ascomycetous, basidiomycetous, and oomycete fungi. Cercosporamide exhibited strong antifungal activities against two pepper anthracnose pathogens, and with EC values of 3.8 and 7.0 µg/mL, respectively. This study suggests the potential application of cercosporamide as an effective antifungal agent in controlling anthracnose in pepper.
一种内生真菌，NG-25菌株，能产生一组结构相关的聚酮化合物，包括尾孢酰胺、拟茎点霉二酮和松萝酸，其中，尾孢酰胺据报道具有很强的抗真菌和抗癌活性。在本研究中，NG-25菌株在七种生长培养基中培养，以确定有利于尾孢酰胺产生的最佳培养条件。在牛肉蛋白胨葡萄糖（BPD）肉汤培养基中培养至第18天时，尾孢酰胺产量达到峰值。在BPD中，尾孢酰胺的效价平均达到77.5μg/mL。纸片扩散法显示，以尾孢酰胺为主要成分的培养滤液抑制了包括子囊菌、担子菌和卵菌在内的多种植物病原体的生长。尾孢酰胺对两种辣椒炭疽病菌表现出很强的抗真菌活性，对辣椒炭疽病菌和辣椒炭疽病菌的EC值分别为3.8和7.0μg/mL。本研究表明尾孢酰胺作为一种有效的抗真菌剂在控制辣椒炭疽病方面具有潜在应用价值。
