Castanho Fernanda Machado, Costa Beatriz Lorena Comin da, Abe Valéria Yukari, Yokoyama Alessandra, Darben Luana Mieko, Oliveira Liliane Santana, Ferreira Everton Geraldo Capote, Lopes Ivani de Oliveira Negrão, Carvalho Mayra Costa da Cruz Gallo de, Balbi-Peña Maria Isabel, Marcelino-Guimarães Francismar Corrêa
Centro de Ciências Biológicas, Programa de Pós-Graduação em Genética e Biologia Molecular, Londrina, PR, Brazil.
Centro de Ciências Agrárias, Programa de Pós-Graduação em Agronomia, Londrina, PR, Brazil.
Genet Mol Biol. 2024 Sep 2;47(3):e20230192. doi: 10.1590/1678-4685-GMB-2023-0192. eCollection 2024.
Effector proteins in Phakopsora pachyrhizi (Pp), the causative agent of Asian Soybean rust, are involved in the infection process. A previous study identified a rust effector Egh16-like family based expression profile during the interaction with soybean. Herein, we scrutinized available the Pp genomes to validate the predicted Egh16-like family of Pp and identify new family members. We described 22 members of the Egh16-like gene family in the Pp MT2006 genome and 18 in the UFV02 and K8108 genomes, highlighting a family expansion. Family members have a small signal peptide, conserved cysteine-rich R/Y/FxC motifs in the C-terminal region, and a virulence-related Egh16-like domain and were able to suppress PTI related responses in Benthamiana. Phylogenetic analysis placed the family members into eight clusters, with members induced during the early stages of rust infection. Members of clusters VI and VII are present in different copy numbers in Pp genomes and suppressed PAMP-related responses.
亚洲大豆锈病的病原体——大豆锈菌（Phakopsora pachyrhizi，简称Pp）中的效应蛋白参与了感染过程。先前的一项研究根据与大豆相互作用期间的表达谱鉴定出一个锈菌效应蛋白Egh16样家族。在此，我们仔细研究了现有的Pp基因组，以验证预测的Pp的Egh16样家族并鉴定新的家族成员。我们在Pp MT2006基因组中描述了22个Egh16样基因家族成员，在UFV02和K8108基因组中描述了18个，突出了该家族的扩增。家族成员具有一个小信号肽，在C末端区域有保守的富含半胱氨酸的R/Y/FxC基序，以及一个与毒力相关的Egh16样结构域，并且能够抑制本氏烟草中与PTI相关的反应。系统发育分析将家族成员分为八个簇，其中成员在锈菌感染早期被诱导。簇VI和VII的成员在Pp基因组中以不同的拷贝数存在，并抑制了与PAMP相关的反应。
