Sysmex 血液分析仪在疟疾检测中的诊断作用：系统评价和荟萃分析。

Diagnostic role of Sysmex hematology analyzer in the detection of malaria: A systematic review and meta-analysis.

机构信息

Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.

Department of Medical Laboratory Sciences, Asrat Woldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia.

出版信息

PLoS One. 2024 Sep 6;19(9):e0296766. doi: 10.1371/journal.pone.0296766. eCollection 2024.

DOI:10.1371/journal.pone.0296766

PMID:39240990

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11379208/

Abstract

BACKGROUND

Malaria control depends primarily on rapid and accurate diagnosis followed by successful treatment. Light microscopy is still used as a gold standard method for the diagnosis of malaria. The Sysmex hematology analyzer is a novel method for malaria detection. Therefore, the aim of this review was to investigate the diagnostic accuracy of the Sysmex hematology analyzer for malaria diagnosis.

METHODS

Electronic databases like PubMed, PubMed Central, Science Direct databases, Google Scholar, and Scopus were used to find relevant articles from April to June 14, 2023. The studies' methodological quality was assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 tool. Using Review Manager 5.4.1, the estimates of sensitivity and specificity, as well as their 95% confidence intervals, were shown in forest plots. Midas software in Stata 14.0 was utilized to calculate the summary estimates of sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio. Heterogeneity was assessed by using I2 statistics. In addition, publication bias was assessed using a funnel plot and Deeks' test. Sub-group and meta- regression analysis were also performed.

RESULTS

A total of 15 studies were assessed for diagnostic accuracy. The sensitivity and specificity of Sysmex hematology analyzer for studies ranged from 46% to 100% and 81% to 100%, respectively. The summary estimate of sensitivity and specificity of Sysmex hematology analyzer were 95% (95% CI: 85%-99%) and 99% (95% CI: 97%-100%), respectively. It had excellent diagnostic accuracy. There were significant heterogeneity among the studies included in this meta-analysis. The summary estimate of sensitivity and specificity of Sysmex hematology analyzer using polymerase chain reaction as the gold standard was 97.6% (95% CI: 83.2, 99.7) and 99.4% (98.5, 99.8), respectively.

CONCLUSION

In this review, Sysmex hematology analyzer had excellent diagnostic accuracy. Therefore, it could be used as an alternate diagnostic tool for malaria diagnosis in the hospital and health center.

TRIAL REGISTRATION

Systematic review registration PROSPERO (2023: CRD42023427713). https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023427713.

摘要

背景

疟疾控制主要依赖于快速准确的诊断，然后进行成功的治疗。显微镜检查仍然是疟疾诊断的金标准方法。Sysmex 血液分析仪是一种新的疟疾检测方法。因此，本综述的目的是调查 Sysmex 血液分析仪对疟疾诊断的诊断准确性。

方法

从 2023 年 4 月至 6 月 14 日，我们使用电子数据库（如 PubMed、PubMed Central、Science Direct 数据库、Google Scholar 和 Scopus）搜索相关文章。使用诊断准确性研究质量评估工具 2（Quality Assessment of Diagnostic Accuracy Studies-2）评估研究的方法学质量。使用 Review Manager 5.4.1，以森林图形式展示灵敏度和特异性及其 95%置信区间的估计值。在 Stata 14.0 中使用 Midas 软件计算灵敏度、特异性、阳性似然比、阴性似然比和诊断比值比的汇总估计值。使用 I2 统计量评估异质性。此外，还使用漏斗图和 Deeks 检验评估发表偏倚。还进行了亚组和元回归分析。

结果

共有 15 项研究评估了诊断准确性。Sysmex 血液分析仪对研究的灵敏度和特异性范围分别为 46%至 100%和 81%至 100%。Sysmex 血液分析仪的灵敏度和特异性的汇总估计值分别为 95%（95%CI：85%-99%）和 99%（95%CI：97%-100%）。它具有出色的诊断准确性。纳入的这项荟萃分析存在显著的异质性。使用聚合酶链反应作为金标准的 Sysmex 血液分析仪的灵敏度和特异性的汇总估计值分别为 97.6%（95%CI：83.2，99.7）和 99.4%（98.5，99.8）。