Seeger W, Lepper H, Wolf H R, Neuhof H
Biochim Biophys Acta. 1985 Jun 14;835(1):58-67. doi: 10.1016/0005-2760(85)90030-x.
Alveolar surfactant is known to be impaired after inhalation of various oxidizing agents (NO2, ozone) as well as in inflammatory lung processes, in which leucocyte-derived active oxygen species or arachidonic acid oxygenation products may be involved. The effect of lipid peroxidation, oxygen-free radicals and oxygenated versus native arachidonic acid on the surface tension behaviour of natural surfactant was tested in vitro. The studies were performed on pooled surfactant material, obtained from bronchoalveolar lavage of rabbit lungs, in a Langmuir trough/Wilhelmy balance system. Initiation of lipid peroxidation with FeCl3/ascorbate or UV radiation and the generation of OH.(FeCl2/EDTA/H2O2), O2-. (xanthine/xanthine oxidase) and 1O2 (NaOCl/H2O2) provoked a common profile of changes: delayed reduction of surface tension during compression with an increase in minimal compressibility accelerated decrease of film pressure during expansion, reduction of hysteresis area and markedly augmented monolayer collapse rate. Addition of arachidonic acid resulted in decreased minimal compressibility, stability index and hysteresis area. Incubation with the arachidonic acid cyclooxygenase products, prostaglandin E2, I2, F2 alpha or thromboxane B2, with soybean lipoxygenase or with H2O2 and O2-exposure caused only moderate or no alteration of surfactant behaviour in vitro.
oxidative stress, but not arachidonic acid oxygenation products, provoked altered surface tension behaviour of natural surfactant in vitro.
已知吸入各种氧化剂（二氧化氮、臭氧）后以及在炎症性肺过程中肺泡表面活性物质会受损，在炎症性肺过程中可能涉及白细胞衍生的活性氧或花生四烯酸氧化产物。在体外测试了脂质过氧化、氧自由基以及氧化型与天然型花生四烯酸对天然表面活性剂表面张力行为的影响。研究是在Langmuir槽/威廉米天平系统中，对从兔肺支气管肺泡灌洗获得的混合表面活性剂材料进行的。用FeCl3/抗坏血酸或紫外线辐射引发脂质过氧化以及生成OH·（FeCl2/EDTA/H2O2）、O2-·（黄嘌呤/黄嘌呤氧化酶）和1O2（NaOCl/H2O2）会引发共同的变化特征：压缩过程中表面张力延迟降低，最小压缩性增加，膨胀过程中膜压力加速降低，滞后面积减小，单层塌陷率显著增加。添加花生四烯酸导致最小压缩性、稳定性指数和滞后面积降低。用花生四烯酸环氧化酶产物、前列腺素E2、I2、F2α或血栓素B2、大豆脂氧合酶或H2O2和O2暴露进行孵育，在体外仅引起表面活性剂行为的中度改变或无改变。
氧化应激而非花生四烯酸氧化产物在体外引发天然表面活性剂表面张力行为改变。
