Mills E L, Björksteń B, Quie P G
Pediatr Res. 1979 Dec;13(12):1341-4. doi: 10.1203/00006450-197912000-00008.
Neonatal susceptibility to overwhelming bacterial infection is commonly attributed to a relative deficiency in serum opsonic activity. However, few studies have compared the functional capacity of the classical complement pathway with that of the alternative complement pathway in the neonate. The opsonic activity of nine maternal infant serum pairs were studied by determining percent uptake of radiolabeled Escherichia coli. Seven mother-infant paired sera were studied using E. coli strains known to be opsonized via the alternative complement pathway: the mean percent uptake of E. coli opsonized in neonatal sera was 16.8%; of those opsonized in maternal sera, 54%; and of those opsonized in control sera, 45% (P less than 0.005). Two E. coli strains requiring the classical complement pathway for opsonization were phagocytized equally well in maternal and infant sera of seven mother-infant pairs. Determination of anti-O hemagglutination inhibition (HI) antibody titers in six maternal sera for one classical complement pathway activating and one alternative complement pathway strain showed no correlation between percent phagocytosis and HI antibody titer. These data would suggest that serum levels of classical pathway components are probably adequate for opsonization of E. coli via the classical pathway, but that low alternative complement pathway activity in neonatal sera may contribute to the newborn's increased susceptibility to bacterial sepsis.
新生儿易受严重细菌感染通常归因于血清调理活性相对不足。然而，很少有研究比较新生儿经典补体途径与替代补体途径的功能能力。通过测定放射性标记大肠杆菌的摄取百分比，研究了9对母婴血清的调理活性。使用已知通过替代补体途径被调理的大肠杆菌菌株研究了7对母婴配对血清：新生儿血清中被调理的大肠杆菌的平均摄取百分比为16.8%；母亲血清中被调理的为54%；对照血清中被调理的为45%（P小于0.005）。7对母婴的母婴血清中，两种需要经典补体途径进行调理的大肠杆菌菌株的吞噬情况相同。对一种经典补体途径激活菌株和一种替代补体途径菌株的6份母亲血清进行抗O血凝抑制（HI）抗体滴度测定，结果显示吞噬百分比与HI抗体滴度之间无相关性。这些数据表明，经典途径成分的血清水平可能足以通过经典途径对大肠杆菌进行调理，但新生儿血清中替代补体途径活性较低可能导致新生儿对细菌性败血症易感性增加。
