Björkstén B, Bortolussi R, Gothefors L, Quie P G
J Pediatr. 1976 Dec;89(6):892-7. doi: 10.1016/s0022-3476(76)80592-6.
Twenty-eight strains of E. coli isolated from infants were compared with respect to opsonic requirements, sensitivity to serum, and ability to activate serum chemotactic factors. Six of the strains were isolated from stools of healthy newborn infants; 22 were isolated from the cerebrospinal fluid or blood of infants with meningitis and/or septicemia. Eighteen of the strains had K1 polysaccharide antigen. Fourteen of the strains (seven with K1 antigen) activated complement via the alternative pathway and all of these strains were well opsonized in 4% pooled human serum. A higher concentration of serum was necessary to opsonize 12 of the 14 strains that did not activate the alternative pathway. A wide variation was also found in opsonic requirements of E. coli strains isolated from healthy and sick infants. There was no relationship of the K1 antigen to opsonic requirements, to capacity to activate complement via the alternative pathway, to generation of chemotactic factors, or to sensitivity to serum cidal activity. Therefore, the association of E. coli with K1 antigen and neonatal meningitis did not appear to be related to these bacteria-serum interactions.
对从婴儿中分离出的28株大肠杆菌进行了比较，涉及调理素需求、对血清的敏感性以及激活血清趋化因子的能力。其中6株从健康新生儿粪便中分离得到；22株从患有脑膜炎和/或败血症婴儿的脑脊液或血液中分离得到。18株菌株具有K1多糖抗原。14株菌株（7株带有K1抗原）通过替代途径激活补体，并且所有这些菌株在4%混合人血清中均能被良好调理。对于另外14株未激活替代途径的菌株，需要更高浓度的血清才能将其中12株调理。从健康和患病婴儿中分离出的大肠杆菌菌株在调理素需求方面也存在很大差异。K1抗原与调理素需求、通过替代途径激活补体的能力、趋化因子的产生或对血清杀菌活性的敏感性均无关联。因此，大肠杆菌与K1抗原和新生儿脑膜炎的关联似乎与这些细菌 - 血清相互作用无关。
