Gomide José A L, Moço Anna C R, Tanaka Leonardo T R, Alexandre Maikon R A, Roca Tárcio Peixoto, Vieira Dall'acqua Deusilene Souza, Costa Nunes Soares Márcia M, Oliveira Ronaldo J, Lima Renata C, Madurro João M, Brito-Madurro Ana G
Institute of Biotechnology, Federal University of Uberlandia, Uberlândia, Brazil.
Institute of Chemistry, Federal University if Uberlandia, Uberlândia, Brazil.
Bioelectrochemistry. 2025 Feb;161:108798. doi: 10.1016/j.bioelechem.2024.108798. Epub 2024 Aug 17.
Coronavirus disease (COVID-19) is caused by infection with the SARS-CoV-2 virus, having already caused more than seven million deaths worldwide. Conventional techniques for SARS-CoV-2 detection have limitations, as high cost, low specificity, and longer analysis time, among others. Biosensors emerge as a necessary alternative to overcome the difficulties of current diagnostics. This paper reports a sensor platform where silver-doped zinc oxide nanomaterial (Ag:ZnONp) was used onto carbon screen-printed electrode and ethidium bromide as indicator for development of a specific electrochemical genosensor for COVID-19. This genosensor demonstrated good linearity between the concentrations of 5.62 × 10-5.62 copies/mL and a detection limit of 5 copies/mL with gRNA in patient's samples, with a response time within 30 min. Molecular modeling and morphological analysis are in agreement with obtained electrochemical results. Additional techniques such as AFM, SEM, and EIS were conducted to characterize the morphological and electrochemical properties of the biosensor's surface. The biosensor was also capable of detecting the target presence in spiked samples and demonstrated a stability of 60 days, higher than other similar biosensors for SARS-CoV-2.
冠状病毒病（COVID-19）由感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起，已在全球造成超过700万人死亡。SARS-CoV-2检测的传统技术存在局限性，如成本高、特异性低、分析时间长等。生物传感器作为克服当前诊断困难的必要替代方案应运而生。本文报道了一种传感器平台，其中将银掺杂氧化锌纳米材料（Ag:ZnONp）用于碳丝网印刷电极，并使用溴化乙锭作为指示剂，以开发用于COVID-19的特异性电化学基因传感器。该基因传感器在患者样本中5.62×10⁻⁵.62拷贝/毫升至5拷贝/毫升的浓度之间表现出良好的线性，检测限为5拷贝/毫升，响应时间在30分钟内。分子建模和形态分析与获得的电化学结果一致。还进行了原子力显微镜（AFM）、扫描电子显微镜（SEM）和电化学阻抗谱（EIS）等其他技术来表征生物传感器表面的形态和电化学性质。该生物传感器还能够检测加标样本中的目标物存在，并表现出60天的稳定性，高于其他类似的SARS-CoV-2生物传感器。
