Shishegari Niusha, Tadjarodi Azadeh, Omidinia Eskandar
Research Laboratory of Inorganic Materials Synthesis, Department of Chemistry, Iran University of Science and Technology (IUST), 16846-13114, Tehran, Iran.
Research Laboratory of Inorganic Materials Synthesis, Department of Chemistry, Iran University of Science and Technology (IUST), 16846-13114, Tehran, Iran.
Talanta. 2025 May 15;287:127662. doi: 10.1016/j.talanta.2025.127662. Epub 2025 Jan 29.
The emergence of COVID-19 has underscored an urgent demand to develop an innovative, rapid, and reliable diagnostic tool for early detection of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Biosensors present a viable alternative, offering reliability, precision, and cost efficiency that address the limitations of current molecular and serological detection methods, thus facilitating timely identification of COVID-19. In this study, a novel nano-genosenor platform fabricated using advanced nanomaterials based on Ce-metal organic framework (Ce-MOF), dendritic palladium nano-structure (Den-PdNS), and sulfur-doped reduced graphene oxide (S-rGO) for detection of RNA-dependent RNA polymerase (RdRp) SARS-CoV-2 gene targets. The fabricated nano-genosensor represents a remarkable limit of detection (LOD) of 0.2 fM, a proper sensitivity of 10.067 μA cm, and a wide linear range from 10 fM to 10 μM. The nano-genosensor's performance was assessed in real saliva samples, demonstrating robust recovery and accuracy, even in complex biological environments, underscoring its potential for medical applications. The fabricated nano-genosensor exhibited proper selectivity, repeatability, and reproducibility in detection of SARS-CoV-2.
新型冠状病毒肺炎（COVID-19）的出现凸显了开发一种创新、快速且可靠的诊断工具以早期检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的迫切需求。生物传感器提供了一种可行的替代方案，具有可靠性、精确性和成本效益，可解决当前分子和血清学检测方法的局限性，从而有助于及时识别COVID-19。在本研究中，一种新型纳米基因传感器平台被制造出来，该平台使用了基于铈金属有机框架（Ce-MOF）、树枝状钯纳米结构（Den-PdNS）和硫掺杂还原氧化石墨烯（S-rGO）的先进纳米材料，用于检测RNA依赖性RNA聚合酶（RdRp）SARS-CoV-2基因靶点。所制造的纳米基因传感器具有显著的检测限（LOD），为0.2 fM，灵敏度为10.067 μA cm，线性范围从10 fM到10 μM。该纳米基因传感器的性能在实际唾液样本中进行了评估，即使在复杂的生物环境中也显示出强大的回收率和准确性，突出了其在医学应用中的潜力。所制造的纳米基因传感器在检测SARS-CoV-2时表现出适当的选择性、重复性和再现性。
