Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Laboratory of Biotechnology, Agriculture Research Center (ARC), Department of Microbiology, Animal Health Research Institute (AHRI), Zagazig, Egypt.
Open Vet J. 2024 Jan;14(1):416-427. doi: 10.5455/OVJ.2024.v14.i1.38. Epub 2024 Jan 31.
Acute lung injury (ALI) is a severe condition distinguished by inflammation and impaired gas exchange in the lungs. a common bacterium, can cause ALI through its virulence factors. is a medicinal plant that has been traditionally used to treat a variety of illnesses due to its anti-inflammatory properties. Chitosan nanoparticles are biocompatible and totally biodegradable materials that have shown potential in drug delivery systems.
To explore the antibacterial activity of -loaded chitosan nanoparticles (AV-CS-NPs) against and with advanced techniques.
The antibacterial efficacy of AV-CS-NPs was evaluated through a broth microdilution assay. In addition, the impact of AV-CS-NPs on induced ALI in rats was examined by analyzing the expression of genes linked to inflammation, oxidative stress, and apoptosis. Furthermore, rat lung tissue was scanned histologically. The rats were divided into three groups: control, ALI, and treatment with AV-CS-NPs.
The AV-CS-NPs that were prepared exhibited clustered semispherical and spherical forms, having an average particle size of approximately 60 nm. These nanoparticles displayed a diverse structure with an uneven distribution of particle sizes. The maximum entrapment efficiency of 95.5% ± 1.25% was achieved. The obtained findings revealed that The minimum inhibitory concentration and minimum bactericidal concentration values were determined to be 5 and 10 ug/ml, respectively, indicating the potent bactericidal effect of the NPs. Also, infected rats explored upregulation in the mRNA expression of and compared to healthy control groups. AV-CS-NP treatment reverses the case where there was repression in mRNA expression of and compared to -treated rats.
These NPs can serve as potential candidates for the development of alternative antimicrobial agents.
急性肺损伤（ALI）是一种严重的疾病，其特征是肺部炎症和气体交换受损。是一种常见的细菌，可以通过其毒力因子引起 ALI。是一种药用植物，由于其抗炎特性，已被传统用于治疗各种疾病。壳聚糖纳米粒子是生物相容和完全可生物降解的材料，在药物传递系统中显示出了潜力。
用先进的技术探索载 AV 的壳聚糖纳米粒子（AV-CS-NPs）对 和 的抗菌活性。
通过肉汤微量稀释法评估 AV-CS-NPs 的抗菌功效。此外，通过分析与炎症、氧化应激和细胞凋亡相关的基因表达，研究了 AV-CS-NPs 对诱导的大鼠 ALI 的影响。此外，还对大鼠肺组织进行了组织学扫描。大鼠分为三组：对照组、ALI 组和 AV-CS-NPs 治疗组。
所制备的 AV-CS-NPs 呈现出聚集的类半球形和球形形态，平均粒径约为 60nm。这些纳米粒子表现出不同的结构，粒径分布不均匀。最大包封效率为 95.5%±1.25%。获得的结果表明，最小抑菌浓度和最小杀菌浓度值分别为 5 和 10μg/ml，表明 NPs 具有很强的杀菌作用。此外，与健康对照组相比， 感染的大鼠的 基因和 基因的 mRNA 表达上调。与 处理的大鼠相比，AV-CS-NP 治疗逆转了 基因和 基因的 mRNA 表达抑制的情况。
这些 NPs 可以作为开发替代抗菌药物的潜在候选物。
