Ramezani Helia, Sazegar Hossein, Rouhi Leila
Department of Biology, ShK.C., Islamic Azad University, Shahrekord, Iran.
Sci Rep. 2025 Jul 1;15(1):22047. doi: 10.1038/s41598-025-06873-2.
Pseudomonas aeruginosa (P. aeruginosa) is a predominant etiological agent of nosocomial infections. This research examined the efficacy of rutin encapsulated in chitosan nanoparticles (Rut-CS) in inhibiting the proliferation of multidrug-resistant P. aeruginosa and diminishing biofilm formation. SEM, DLS and FTIR analyzed the nanoparticles to ascertain their shape. The antibacterial activity was evaluated using agar well diffusion. The findings indicated that Rut-CS nanoparticles had considerable antibacterial efficacy and substantially reduced the expression of critical biofilm-associated genes, lasR and AlgD. The Rut-CS nanoparticles exhibited no toxicity to human dermal fibroblast (HDF) cells at the minimal inhibitory dose. The data indicate that Rut-CS nanoparticles may serve as a viable technique to address highly resistant P. aeruginosa infections, facilitating novel antibacterial research methodologies.
铜绿假单胞菌（P. aeruginosa）是医院感染的主要病原体。本研究考察了壳聚糖纳米粒包裹的芦丁（Rut-CS）抑制多重耐药铜绿假单胞菌增殖和减少生物膜形成的效果。通过扫描电子显微镜（SEM）、动态光散射（DLS）和傅里叶变换红外光谱（FTIR）分析纳米粒以确定其形状。采用琼脂孔扩散法评估抗菌活性。研究结果表明，Rut-CS纳米粒具有显著的抗菌效果，并大幅降低了关键生物膜相关基因lasR和AlgD的表达。在最小抑菌剂量下，Rut-CS纳米粒对人皮肤成纤维细胞（HDF）无毒性。数据表明，Rut-CS纳米粒可能是解决高度耐药铜绿假单胞菌感染的一种可行技术，有助于开展新的抗菌研究方法。
