College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, Hangzhou, China.
OIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Virulence. 2022 Dec;13(1):1304-1314. doi: 10.1080/21505594.2022.2103233.
serotype 2 (SS2), an emerging zoonotic pathogen, causes swine diseases and human cases of streptococcal toxic shock syndrome. RNA-binding proteins (RBPs) can modulate gene expression through post-transcriptional regulation. In this study, we identified an RBP harbouring an S1 domain, named RbpA, which facilitated SS2 adhesion to host epithelial cells and contributed to bacterial pathogenicity. Comparative proteomic analysis identified 145 proteins that were expressed differentially between Δ strain and wild-type strain, including several virulence-associated factors, such as the extracellular protein factor (EF), SrtF pilus, IgA1 protease, SBP2 pilus, and peptidoglycan-binding LysM' proteins. The mechanisms underlying the regulatory effects of RbpA on their encoding genes were explored, and it was found that RbpA regulates gene expression through diverse mechanisms, including post-transcriptional regulation, and thus acts as a global regulator. These results partly reveal the pathogenic mechanism mediated by RbpA, improving our understanding of the regulatory systems of and providing new insights into bacterial pathogenicity.
血清型 2（SS2）是一种新兴的人畜共患病病原体，可引起猪病和人类链球菌中毒性休克综合征病例。RNA 结合蛋白（RBPs）可以通过转录后调控来调节基因表达。在本研究中，我们鉴定了一种含有 S1 结构域的 RBP，命名为 RbpA，它促进 SS2 黏附宿主上皮细胞，并有助于细菌的致病性。比较蛋白质组学分析鉴定了 145 种在Δ株和野生型菌株之间表达差异的蛋白，其中包括几种毒力相关因子，如细胞外蛋白因子（EF）、SrtF 菌毛、IgA1 蛋白酶、SBP2 菌毛和肽聚糖结合 LysM' 蛋白。还探索了 RbpA 对其编码基因的调控作用的机制，发现 RbpA 通过多种机制（包括转录后调控）调节基因表达，因此作为一种全局调节因子发挥作用。这些结果部分揭示了 RbpA 介导的致病机制，增进了我们对调控系统的理解，并为细菌的致病性提供了新的见解。
