Laitman Benjamin M, Charytonowicz Daniel, Zhu Ashley J, Lynch Katie, Varelas Eleni A, Burton Madeline, Andreou Christina, Kore Pragati, Kirke Diana N, Chen Ya-Wen, Beaumont Kristin G, Sebra Robert, Genden Eric M, Courey Mark S
Department of Otolaryngology, Icahn School of Medicine at Mount Sinai, New York, New York, 10029, U.S.A.
Icahn School of Medicine at Mount Sinai, New York, New York, 10029, U.S.A.
Laryngoscope. 2024 Jul;134(7):3193-3200. doi: 10.1002/lary.31334. Epub 2024 Feb 28.
The function of the vocal folds (VFs) is determined by the phenotype, abundance, and distribution of differentiated cells within specific microenvironments. Identifying this histologic framework is crucial in understanding laryngeal disease. A paucity of studies investigating VF cellular heterogeneity has been undertaken. Here, we examined the cellular landscape of human VFs by utilizing single-nuclei RNA-sequencing.
Normal true VF tissue was excised from five patients undergoing pitch elevation surgery. Tissue was snap frozen in liquid nitrogen and subjected to cellular digestion and nuclear extraction. Nuclei were processed for single-nucleus sequencing using the 10X Genomics Chromium platform. Sequencing reads were assembled using cellranger and analyzed with the scanpy package in python.
RNA sequencing revealed 18 global cell clusters. While many were of epithelial origin, expected cell types, such as fibroblasts, immune cells, muscle cells, and endothelial cells were present. Subcluster analysis defined unique epithelial, immune, and fibroblast subpopulations.
This study evaluated the cellular heterogeneity of normal human VFs by utilizing single-nuclei RNA-sequencing. With further confirmation through additional spatial sequencing and microscopic imaging, a novel cellular map of the VFs may provide insight into new cellular targets for VF disease.
NA Laryngoscope, 134:3193-3200, 2024.
声带（VFs）的功能由特定微环境中分化细胞的表型、丰度和分布决定。识别这种组织学框架对于理解喉部疾病至关重要。目前针对声带细胞异质性的研究较少。在此，我们利用单核RNA测序来研究人类声带的细胞图谱。
从五名接受音调升高手术的患者身上切除正常的真声带组织。将组织在液氮中速冻，然后进行细胞消化和细胞核提取。使用10X基因组学铬平台对细胞核进行单核测序处理。测序读数使用cellranger进行组装，并在Python中使用scanpy软件包进行分析。
RNA测序揭示了18个整体细胞簇。虽然许多细胞簇起源于上皮细胞，但也存在成纤维细胞、免疫细胞、肌肉细胞和内皮细胞等预期的细胞类型。亚群分析定义了独特的上皮细胞、免疫细胞和成纤维细胞亚群。
本研究利用单核RNA测序评估了正常人声带的细胞异质性。通过进一步的空间测序和显微镜成像进行确认，声带的新细胞图谱可能为声带疾病提供新的细胞靶点。
NA 喉镜，134:3193 - 3200，2024年。
