Vaz W L, Hallmann D, Clegg R M, Gambacorta A, De Rosa M
Eur Biophys J. 1985;12(1):19-24. doi: 10.1007/BF00254091.
We have used the fluorescence recovery after photobleaching technique to study the translational diffusion, in L alpha phase multibilayers of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), of fluorescent derivatives of 1-palmitoyl-2-oleoylphosphatidylethanolamine (NBD-POPE) and a membrane-spanning phosphatidylethanolamine (NBD-MSPE). The latter derivative was prepared from a membrane-spanning glycerol-dialkyl-glycerol tetraether lipid isolated from the thermophilic and acidophilic archaebacterium Sulfolobus solfataricus. The translational diffusion was examined between about 15 degrees and 45 degrees C. It is shown that over this temperature range the translational diffusion coefficient for NBD-MSPE is approximately 2/3 that for NBD-POPE which spans only one monolayer of the bilayer. The result is interpreted in terms of existing models for translational diffusion in lipid membranes.
我们使用了光漂白后荧光恢复技术，来研究1-棕榈酰-2-油酰磷脂酰乙醇胺（NBD-POPE）的荧光衍生物以及一种跨膜磷脂酰乙醇胺（NBD-MSPE）在1-棕榈酰-2-油酰磷脂酰胆碱（POPC）的Lα相多层膜中的平移扩散。后一种衍生物是由从嗜热嗜酸古细菌嗜热栖热菌中分离出的跨膜甘油二烷基甘油四醚脂质制备而成。在大约15摄氏度至45摄氏度之间检测了平移扩散。结果表明，在这个温度范围内，NBD-MSPE的平移扩散系数约为仅跨越双层膜一个单层的NBD-POPE的平移扩散系数的2/3。根据脂质膜中平移扩散的现有模型对该结果进行了解释。
