Rao S A, Dewanjee M K
Eur J Nucl Med. 1982;7(6):282-5. doi: 10.1007/BF00251484.
A rabbit red cell model was used to determine the cell labeling properties of three lipid-soluble 111In-complexes: 111In-oxine, 111In-acetylacetone, and 111In-tropolone. Partition coefficients (olive oil/buffer) were measured to determine the lipid solubility and were 3.54, 7.93, and 18.18 for 111In-oxine, 111In-acetylacetone, and 111In-tropolone respectively. The effect of the concentration of these three chelating agents on labeling efficiencies was studied. The factors influencing the labeling efficiencies of these complexes such as cell density, time of incubation, influence of temperature, pH, effect of plasma proteins, and citrate ion concentration in the cell-labeling medium were studied. Labeling yields as high as 95.15 +/- 4.15% were achieved with 111In-tropolone after a 10-min incubation at 37 degrees C. The optimum pH for cell labeling was 6.5. Excess citrate ion (greater than 3.02 mg/ml) and small amounts of plasma proteins (greater than 10 microliter/ml) decreased the labeling efficiencies in all three cases. Distribution of these 111In-complexes in membrane, membrane fragments, and hemoglobin was studied after hemolysis. In spite of the higher lipid solubility of 111In-tropolone, the transchelation capacity appears to be similar to that of 111In-oxine. 111In-acetylacetone had the highest transchelation capacity.
采用兔红细胞模型来测定三种脂溶性铟 - 111配合物的细胞标记特性：铟 - 111 - 8 - 羟基喹啉、铟 - 111 - 乙酰丙酮和铟 - 111 - 托酚酮。通过测量分配系数（橄榄油/缓冲液）来确定脂溶性，铟 - 111 - 8 - 羟基喹啉、铟 - 111 - 乙酰丙酮和铟 - 111 - 托酚酮的分配系数分别为3.54、7.93和18.18。研究了这三种螯合剂的浓度对标记效率的影响。研究了影响这些配合物标记效率的因素，如细胞密度、孵育时间、温度影响、pH值、血浆蛋白的影响以及细胞标记培养基中柠檬酸根离子浓度。在37℃孵育10分钟后，铟 - 111 - 托酚酮的标记产率高达95.15±4.15%。细胞标记的最佳pH值为6.5。在所有三种情况下，过量的柠檬酸根离子（大于3.02mg/ml）和少量的血浆蛋白（大于10μl/ml）都会降低标记效率。溶血后研究了这些铟 - 111配合物在膜、膜碎片和血红蛋白中的分布。尽管铟 - 111 - 托酚酮的脂溶性较高，但其转螯合能力似乎与铟 - 111 - 8 - 羟基喹啉相似。铟 - 111 - 乙酰丙酮具有最高的转螯合能力。
