She Jiarong, Li Qingyang, Cui Maokai, Zheng Qiong, Yang Jie, Chen Tiantian, Shen Danyu, Peng Shaofeng, Li Chi, Liu Yihua
Hunan Academy of Forestry, Changsha, China.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, China.
Front Nutr. 2024 Aug 23;11:1440279. doi: 10.3389/fnut.2024.1440279. eCollection 2024.
Less research has been conducted on the association between camellia oil's (CO) phenolic composition and antioxidant capability. In this study, the phenolic profile of CO and its connection to antioxidant capacity were examined utilizing a combination of widely-targeted phenolic metabolomics and multivariate statistical analysis. A total of 751 phenolics were discovered. The WGCNA was used to link phenols to antioxidants, yielding 161 antioxidant-related phenols from the blue module. In response to several antioxidant assays, 59 (FRAP), 59 (DPPH), and 53 (ABTS) phenolics were identified as differential phenolic markers (DPMs). Further stepwise multiple linear regression revealed six DPMs that substantially influenced the antioxidant capacities. Nine metabolic pathways and their associated network mechanisms for the most significant phenolics were developed. This study sheds light on the phenolic content of CO, elucidates their role in antioxidant activity, and lays the groundwork for improving extraction techniques and generating improved product.
关于山茶油（CO）的酚类成分与抗氧化能力之间的关联，所开展的研究较少。在本研究中，利用广泛靶向的酚类代谢组学和多变量统计分析相结合的方法，对CO的酚类概况及其与抗氧化能力的关系进行了研究。共发现了751种酚类物质。利用加权基因共表达网络分析（WGCNA）将酚类物质与抗氧化剂联系起来，从蓝色模块中得到了161种与抗氧化相关的酚类物质。针对几种抗氧化试验，59种（铁还原抗氧化能力法，FRAP）, 59种（1,1-二苯基-2-三硝基苯肼，DPPH）和53种（2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐，ABTS）酚类物质被鉴定为差异酚类标志物（DPMs）。进一步的逐步多元线性回归分析揭示了六种对抗氧化能力有显著影响的DPMs。针对最主要的酚类物质，构建了九条代谢途径及其相关的网络机制。本研究揭示了CO的酚类含量情况，阐明了它们在抗氧化活性中的作用，并为改进提取技术和生产改良产品奠定了基础。
