Hashimura T, Yoshida O
Jpn J Cancer Res. 1985 May;76(5):321-3.
A single topical application of 7,12-dimethylbenz[a]anthracene (DMBA) followed by twice-weekly 12-O-tetradecanoyl phorbol-13-acetate (TPA) treatment was given to the skin of female CD-1 mice, and soft agar colony formation of the epidermal cells was evaluated periodically during the treatment. From the second week, colony growth was observed and the number of colonies increased with time, reaching a significant level from the fourth week. Colony formation was observed earlier than papilloma development in the mouse skin. Soft agar colony-forming assay might be a useful tool to analyze the early events in carcinogenesis.
对雌性CD-1小鼠皮肤单次局部涂抹7,12-二甲基苯并[a]蒽（DMBA），随后每周两次涂抹12-O-十四烷酰佛波醇-13-乙酸酯（TPA）进行处理，并在处理期间定期评估表皮细胞的软琼脂集落形成情况。从第二周开始观察到集落生长，集落数量随时间增加，从第四周起达到显著水平。在小鼠皮肤中，集落形成比乳头状瘤发展出现得更早。软琼脂集落形成试验可能是分析致癌早期事件的有用工具。
