Gorelyshev A S, Kul'ba A M, Tsygankov Iu D, Fomichev Iu K
Genetika. 1985 Apr;21(4):548-55.
Seventy three temperature-sensitive mutants of Pseudomonas aeruginosa SM phage have been obtained using different mutagens and assigned to thirteen complementation groups. Representative mutants of each group have been studied with the aim of characterizing tentatively the time of genes expression in infected bacteria. Two genes appear to function during the first minutes after infection, whereas the remaining genes are needed for late functions. Most of the temperature-sensitive functions in the different mutants are reversible, i.e. they become active when the infected cells are shifted-down to the permissive temperature.
利用不同诱变剂已获得73株铜绿假单胞菌SM噬菌体的温度敏感突变体,并将其分为13个互补群。对每组代表性突变体进行了研究,目的是初步确定感染细菌中基因表达的时间。有两个基因似乎在感染后的最初几分钟发挥作用,而其余基因则是后期功能所必需的。不同突变体中的大多数温度敏感功能是可逆的,即当感染细胞转移到允许温度时它们会变得活跃。