Plotnikova T G, Dzhusupova A B, Khrenova E A, Krylov V N
Genetika. 1982 Nov;18(11):1793-8.
A group of ts mutants of phi KZ phage specific for Pseudomonas aeruginosa was isolated and studied. A phi KZ phage particle has a unique feature: a specific structure as an internal body concerned with DNA packaging which is present in the phage capsid. Ts mutants were distributed into complementation groups. The time of the development block when grown at elevated temperatures was determined for mutants from several groups, the exact stage of the block having been defined for some late ts mutants. As was found for ts759, the stage of connection of heads containing DNA and the inner body with tails was blocked. The results obtained with ts759 allowed to prove the circular location of fibrous material (DNA?) around the inner body. The conditions for phage crosses were revealed. The genetic map of phi KZ is circular. It may be concluded, considering the linear phi KZ DNA structure in capsids, that circular permutations of phage DNA take place during phage maturation or DNA packaging.
分离并研究了一组对铜绿假单胞菌具有特异性的φKZ噬菌体的温度敏感（ts）突变体。φKZ噬菌体颗粒有一个独特的特征：作为与DNA包装有关的内部结构存在于噬菌体衣壳中的特定结构。ts突变体被分为互补群。确定了来自几个组的突变体在高温下生长时发育阻断的时间，对于一些晚期ts突变体已经确定了阻断的确切阶段。如ts759的情况所示，含有DNA的头部和内部结构与尾部连接的阶段被阻断。用ts759获得的结果证明了纤维状物质（DNA？）围绕内部结构呈环状分布。揭示了噬菌体杂交的条件。φKZ的遗传图谱是环状的。考虑到衣壳中φKZ DNA的线性结构，可以得出结论，噬菌体DNA在噬菌体成熟或DNA包装过程中发生环状排列。
