Li Shuhong, He Jiangbo, Wu Qunfu, Gou Jianghui, Wang Donglou, Niu Xuemei
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China.
Kunming Key Laboratory of Respiratory Disease, Kunming University, Kunming, China.
Mycology. 2024 Apr 2;15(3):485-505. doi: 10.1080/21501203.2024.2324993. eCollection 2024.
Thermomyces dupontii harbors two paralogs ( and ) in the gene cluster for the biosynthesis of prenylated indole alkaloids (PIAs) and correponding iron chelators with assigned as one protein containing a CYP like domain fused with a FAD-binding domain-containing oxidoreductase. Genetic manipulation and metabolic profile analysis indicated both and were involved in transforming simple PIAs to their corresponding iron chelators. Moreover, is responsible for bolstering simple PIAs to complex PIAs, and for reinforcing conjugating unsaturated systems in complex PIAs. Chemical investigation led to isolation and characterization of novel complex PIA metabolites with more oxidations. also contributed to forming the third iron-chelating core in iron chelators. A series of iron bioassays and infrastructure analysis revealed that lack of these genes caused strongly elevated Fe levels but attenuated Fe levels, together with abnormal mitochondria in mycelia and lipid droplets and vacuoles in conidia. Phenotype analysis revealed that and facilitated fungal colony pigments, conidial formation and germination via bolstering conidiophores and cell walls in response to temperature reduction.
杜邦嗜热霉菌在异戊烯基化吲哚生物碱（PIAs）生物合成基因簇中含有两个旁系同源基因（ 和 ）以及相应的铁螯合剂，其中 被指定为一种含有与含FAD结合结构域的氧化还原酶融合的CYP样结构域的蛋白质。基因操作和代谢谱分析表明， 和 都参与将简单的PIAs转化为其相应的铁螯合剂。此外， 负责将简单的PIAs提升为复杂的PIAs，而 则负责增强复杂PIAs中不饱和系统的共轭。化学研究导致分离和表征了具有更多氧化作用的新型复杂PIA代谢物。 也有助于在铁螯合剂中形成第三个铁螯合核心。一系列铁生物测定和基础结构分析表明，缺乏这些 基因会导致铁水平大幅升高，但 水平降低，同时菌丝体中线粒体异常，分生孢子中出现脂滴和液泡。表型分析表明， 和 通过在温度降低时增强分生孢子梗和细胞壁来促进真菌菌落色素形成、分生孢子形成和萌发。
