Niaudet B, Jannière L, Ehrlich S D
J Bacteriol. 1985 Jul;163(1):111-20. doi: 10.1128/jb.163.1.111-120.1985.
Linear DNA molecules composed of a central region nonhomologous with the Bacillus subtilis chromosome and two flanking regions homologous with the chromosome can integrate into the chromosome, provided that the homologous regions have the same relative orientation. The resulting chromosome can be maintained in a haploid or in a merodiploid cell together with a parental chromosome. This can most easily be explained by supposing that the integration occurs by crossing over at each homologous region and that a part of the chromosome between these regions is deleted and replaced by the central nonhomologous region of the integrating molecule. If no essential genes were replaced during that process a haploid cell would be obtained; if essential genes were replaced a merodiploid cell would be obtained. The use of appropriate linear molecules therefore should allow the induction of deletions, extending from a given chromosomal site in a predetermined direction, and defined duplications in the B. subtilis chromosome.
由与枯草芽孢杆菌染色体非同源的中央区域和与该染色体同源的两个侧翼区域组成的线性DNA分子，只要同源区域具有相同的相对取向，就可以整合到染色体中。所得染色体可以与亲本染色体一起维持在单倍体细胞或部分二倍体细胞中。最容易解释这种情况的假设是，整合通过在每个同源区域发生交换而发生，并且这些区域之间的一部分染色体被删除，由整合分子的中央非同源区域取代。如果在此过程中没有替换必需基因，将获得单倍体细胞；如果替换了必需基因，将获得部分二倍体细胞。因此，使用合适的线性分子应该能够诱导从给定染色体位点沿预定方向延伸的缺失，以及枯草芽孢杆菌染色体中确定的重复。
