同源DNA重组的效率在枯草芽孢杆菌染色体上各部位有所不同。
Efficiency of homologous DNA recombination varies along the Bacillus subtilis chromosome.
作者信息
Vagner V, Ehrlich S D
机构信息
Institut des Biotechnologies, Centre de Recherche de Jouy en Josas, France.
出版信息
J Bacteriol. 1988 Sep;170(9):3978-82. doi: 10.1128/jb.170.9.3978-3982.1988.
Abstract
Structures consisting of a genetic marker (erythromycin or kanamycin resistance, thymidylate synthetase) flanked by 3.4-kilobase direct repeats (pBR322 sequences) were inserted in 12 different locations of the Bacillus subtilis chromosome. Recombination between the repeats was followed by the loss of the genetic marker. Recombination frequencies found in different locations varied from 1.2 X 10(-5) to 40 X 10(-5) per cell generation. Such differences were highly significant (P less than 0.001).
摘要
由两侧带有3.4千碱基直接重复序列（pBR322序列）的遗传标记（红霉素或卡那霉素抗性、胸苷酸合成酶）组成的结构被插入枯草芽孢杆菌染色体的12个不同位置。重复序列之间的重组伴随着遗传标记的丢失。在不同位置发现的重组频率在每细胞世代1.2×10⁻⁵至40×10⁻⁵之间变化。这种差异非常显著（P小于0.001）。
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